Characterization of Bluetongue Virus Ribonucleic Acid
Journal of VirologyPublished 1 January 1970Open access
D. W. Verwoerd, H. Louw, R. A. Oellermann
Citations199
SJR quartileQ1
SJR score1.28
SNIP0.90
Generate an AI Snapshot to get a quick, structured summary of this paper.
Study Snapshot
ObjectiveStudy objective
MethodsResearch methodology
PopulationPopulation studied
Sample sizeSample sizes
OutcomesStudy outcomes here
ResultsStudy results comes here
LimitationsResearch study limitations comes here
A concise AI-generated summary of the paper will appear here once you click Generate AI Snapshot.
TL;DR
An improved purification procedure yielded bluetongue virus free from any single-stranded ribonucleic acid (RNA) component, and the denaturation of the genome and separation of the resulting fragments are discussed.
Abstract
An improved purification procedure yielded bluetongue virus free from any single-stranded ribonucleic acid (RNA) component. Double-stranded RNA obtained from purified virus or isolated from infected cells was fractionated into 5 components by means of sucrose gradient sedimentation analysis, and into 10 components by electrophoresis on polyacrylamide gels. The size of these components vary from 0.5 x 10(6) to 2.8 x 10(6) daltons, with a total molecular weight estimate of about 1.5 x 10(7) for the viral nucleic acid. The denaturation of the genome and separation of the resulting fragments are also discussed.
Keywords
MedicineAgricultural and Biological Sciences
Analytical BiochemistryA simple efficient liquid scintillator for counting aqueous solutions in a liquid scintillation counter
7,507 Citations1960George A. Bray
A modification of the naphthalene-dioxane-PPO liquid scintillator has been described which will allow up to 3.0 ml of an aqueous solution to be counted, which has been used to count carbon-14 and tritium in urine, plasma, and liver homogenate.
Journal of Molecular BiologySedimentation studies of the size and shape of DNA
2,169 Citations1965F. William Studier
Analytical zone sedimentation ( Vinograd, Bruner, Kent & Weigle, 1963) is shown to be a sensitive and reliable method for detecting differences in conformation and for determining the molecular weight of homogeneous phage DNA's.
Biochemical JournalThe fractionation of high-molecular-weight ribonucleic acid by polyacrylamide-gel electrophoresis
2,105 Citations1967UE Loening
Electrophoresis in 2.2-2.6% gels gives a fractionation similar to density-gradient centrifugation, and the resolution is greater and more detailed than by centrifugations, and many samples can be analysed simultaneously and rapidly.
Biochemical and Biophysical Research CommunicationsSedimentation characteristics of rapidly labelled RNA from HeLa cells
1,030 Citations1962Klaus Scherrer, James Darnell
Analytical BiochemistryTables for estimating sedimentation through linear concentration gradients of sucrose solution
446 Citations1967C.R. McEwen
A set of tables is presented for use in the design and interpretation of centrifuge experiments involving sedimentation in linear sucrose density gradients that can be used to predict the position of a material of given sedimentation coefficient and density.
Journal of VirologySeparation of Ten Reovirus Genome Segments by Polyacrylamide Gel Electrophoresis
282 Citations1968Aaron J. Shatkin, Jean D. Sipe +1 more
Double-stranded ribonucleic acid extracted from purified reoviruses of all three serotypes and from type 3 virus-infected cells was analyzed by polyacrylamide gel electrophoresis and calculated that each RNA includes 10 segments.
Review of Scientific InstrumentsLiquid Scintillator for the Analysis of Tritium in Water
266 Citations1957Frank E. Kinard
Progress in nucleic acid research and molecular biologySome Problems Concerning the Macromolecular Structure of Ribonucleic Acids
207 Citations1963A.S. Spibin
The chapter describes that phenol deproteinization involves the treatment of biological material with water-saturated phenol, provides immediate and practically complete inactivation of nucleases, causes no degradation of polyribonucleotide chains during the isolation procedure, completely removes the proteins, and thus secures a high purity of the RNA preparation.
Journal of Molecular BiologyStudies on reovirus RNA
206 Citations1967A.R. Bellamy, Lucille Shapiro +2 more
It would appear that the reovirus genome does not replicate as one intact molecule, but rather in the form of these same segments, which are likely to be segments of double-stranded RNA.
Journal of Molecular BiologyRegulation of transcription of the reovirus genome
124 Citations1968Y. Watanabe, Stewart Millward +1 more
Inhibition of protein synthesis by addition of cycloheximide at the time of infection permitted only a limited number of the double-stranded viral RNA segments to be transcribed, suggesting that the early messengers might be synthesized by a pre-existing polymerase.
VirologyPurification and characterization of bluetongue virus
117 Citations1969D. W. Verwoerd
Purified bluetongue virus was shown to possess a double-stranded RNA genome, very similar to that of reovirus, consisting of at least three double-Stranded components, reflecting profound differences in morphology.
Journal of VirologyStructural Units of Reovirus Ribonucleic Acid and Their Possible Functional Significance
101 Citations1967Y. Watanabe, Angus Graham
By whatever means this fragment of double-stranded RNA may be joined into the viral RNA molecule, it seems to act as a specific unit for transcription of an uninterrupted messenger RNA of equivalent length.
Biochemical and Biophysical Research CommunicationsThe solvent denaturation of double-stranded RNA from poliovirus infected HeLa cells
91 Citations1966Louis M. Katz, Sheldon Penman
It is shown that the replicative form of poliovirus RNA, which sediments as a single species at 20S can be completely transformed into a more rapidly sedimenting species, whose behavior is the same as single-stranded RNA extracted from mature virus, sedimenting at 35S.
Proceedings of the National Academy of SciencesSingle-stranded, adenine-rich RNA from purified reoviruses.
76 Citations1968Aaron J. Shatkin, Jean D. Sipe
The chiral stationary phase for high-performance liquid chromatography showed good chiral recognition ability towards various racemates, including Na6(CO3)(SO4)2, Na2SO4, and Na2CO3.
Journal of Molecular BiologyElectron microscopy of RNA strands released from individual reovirus particles
60 Citations1968C. Vásquez, Albrecht K. Kleinschmidt
Electron microscopy of virus-protein monolayers showed that each virion expelled about 11 fragments of 8.3 μ, maximum total length, which gives an estimated RNA molecular weight between 17 and 22 million daltons per virion.
UpSpace Institutional Repository (University of Pretoria)A preliminary antigenic classification of strains of bluetongue virus
46 Citations1960Peter Howell
Methods in enzymology on CD-ROM/Methods in enzymology[90a] Chromatographic separation of RNA from DNA on MAK columns
14 Citations1967S. Osawa, Atuhiro Sibatani
This paper presents an example of the fractionation of whole nucleic acids prepared by sodium dodecyl sulfate-phenol method from Escherichia coli cells is given, and shows how a shallow concentration gradient can be employed by appropriately increasing the volume of the eluting solutions.