Collagenase activity in rabbit carcinoma: Cell source and cell interactions
International Journal of CancerPublished 15 March 1983
Mustafa Kh. Dabbous, Mahmoud Eltorky, Lena Haney, Burchada Brinkley, Nahed Sobhy
Citations42
SJR quartileQ1
SJR score2.25
SNIP1.76
Generate an AI Snapshot to get a quick, structured summary of this paper.
Study Snapshot
ObjectiveStudy objective
MethodsResearch methodology
PopulationPopulation studied
Sample sizeSample sizes
OutcomesStudy outcomes here
ResultsStudy results comes here
LimitationsResearch study limitations comes here
A concise AI-generated summary of the paper will appear here once you click Generate AI Snapshot.
TL;DR
Serum from tumor‐bearing rabbits appeared to stimulate the release of enzyme activity in cultures of either cell type and was enhanced by the presence of E‐cells in mixed cultures as well as by medium conditioned by the E‐ cells type.
Abstract
Fibroblast-like cells (F-cells) and epithelial-like (E-cells) derived from cultures of rabbit VX-2 carcinoma released collagenase in both active and latent forms in serum-free medium at a level higher than that of normal rabbit fibroblast cultures. The enhanced capacity of the F-cells to release the enzyme, however, continued only for a few passages and then decreased significantly to a low level similar to that of the normal fibroblasts. The enzyme-release by the E-cells continued for a few more passages at a relatively moderate level, higher than that of normal fibroblasts. The release of collagenase in cultures of F-cells was enhanced by the presence of E-cells in mixed cultures as well as by medium conditioned by the E-cells type. Addition of cytochalasin B at 2 micrograms/ml did not significantly effect the enzyme activity released in the cultures. Serum from tumor-bearing rabbits appeared to stimulate the release of enzyme activity in cultures of either cell type.
Keywords
Biochemistry, Genetics and Molecular Biology
Journal of Biological ChemistryPROTEIN MEASUREMENT WITH THE FOLIN PHENOL REAGENT
318,326 Citations1951OliverH. Lowry, NiraJ. Rosebrough +2 more
Procedures are described for measuring protein in solution or after precipitation with acids or other agents, and for the determination of as little as 0.2 gamma of protein.
NatureMetastatic potential correlates with enzymatic degradation of basement membrane collagen
1,773 Citations1980Lance A. Liotta, Karl Tryggvason +4 more
The cell lines with the highest incidence of spontaneous metastasis exhibit the greatest level of type IV collagen-degrading activity in two different assays using either living cells or media obtained from cell cultures.
JAMALysosomes in Biology and Pathology
892 Citations1974David Lagunoff
This disclaimer cannot diminish the immense importance to biology of de Duve's discovery of the lysosome or, as he might put it, the discovery of de duve's concept of the LYSosome.
The Journal of Experimental MedicineAN ENZYMATIC FUNCTION ASSOCIATED WITH TRANSFORMATION OF FIBROBLASTS BY ONCOGENIC VIRUSES
771 Citations1973Jay C. Unkeless, A. Tobia +4 more
At least one step of the fibrinolytic process shows specificity resembling that of trypsin, and the sera of sarcoma-bearing chickens contain an inhibitor of thefibrinolysin, whereas normal chicken sera do not.
Biochemical and Biophysical Research CommunicationsCollagenase production by human skin fibroblasts
256 Citations1975Eugene A. Bauer, George P. Stricklin +2 more
The presence of this inhibitor of collagenase may account for previous inability to detect collagenase in human skin fibroblast cultures and it is also possible that some of the inactive enzyme exists in the medium in the form of a proenzyme.
PubMedCollagenolytic enzymes in human neoplasms.
178 Citations1972Marc H. Dresden, Stephen A. Heilman +1 more
Tumor enzymes appear to be similar to the collagenases isolated from normal human skin by these criteria, and tumors of the colon and carcinomas of both squamous and basal cell origin displayed a high frequency of activity.
Biochemical JournalA specific collagenase from rabbit fibroblasts in monolayer culture
169 Citations1974Zena Werb, Mary C. Burleigh
Long-term cell cultures originating from rabbit skin or synovium from rabbits with experimentally induced arthritis also secreted specific collagenase, and human fibroblasts released only very small amounts of collagenase.
Biochemical JournalPurification and characterization of a collagenase extracted from rabbit tumours
168 Citations1975Peter McCroskery, J.F. Richards +1 more
A collagenase was purified from homogenates of V2 ascites-cell carcinoma growing in rabbit muscle and studies with zonal density-gradient centrifugation suggested that the enzyme was bound to fibrillar substrate (collagen) extracellularly, but that it was not associated with enzymes originating in cell mitochondria, microsomal preparations or lysosomes.
JNCI Journal of the National Cancer InstituteDegradation of Basement Membrane by Murine Tumor Cells2
156 Citations1977Lance A. Liotta, Jerome Kleinerman +2 more
It was found that tumor cells collected in the tumor venous drainage were associated with a significantly greater solubilization of basement membrane than were tumor cells obtained from the primary tumor mass.
International Journal of CancerRat mammary carcinoma cells secrete active collagenase and activate latent enzyme in the stroma via plasminogen activator
148 Citations1981Robert L. O’Grady, Linda I. Upfold +1 more
Results demonstrate that the neoplastic cells themselves secrete collagenase as an active enzyme, demonstrating that this tumour possesses potent collagenolytic ability in vitro which may be partly responsible for its rapid invasion in vivo.
ScienceTumor Cell Collagenase and Its Inhibition by a Cartilage-Derived Protease Inhibitor
143 Citations1977Klaus E. Kuettner, L. W. Soble +4 more
Both cell cultures secreted collagenase, and this activity was inhibited by a cartilage-derived protein of low molecular weight, which suggests that cartilage is rarely invaded by neoplasms, and may play an important role in the regulation of tumor invasion.
Proceedings of the National Academy of SciencesInhibition of tumor growth, vascularization, and collagenolysis in the rabbit cornea by medroxyprogesterone.
137 Citations1981J Gross, Richard G. Azizkhan +4 more
The data suggest that local hormonal interference with neovascularization, collagenase production, and tumor growth can prevent neoplastic invasion and destruction of a dense collagenous connective tissue.
PubMedCollagenolytic activities of squamous cell carcinoma of the skin.
132 Citations1973Ken Hashimoto, Yuji Yamanishi +3 more
Electron microscopic and physicochemical studies suggest that squamous cell carcinomas of the skin contain specific collagenase that is active in vivo.
Proceedings of the National Academy of SciencesRegulation of corneal collagenase production: epithelial-stromal cell interactions.
117 Citations1978B Johnson-Muller, J Gross
Mixtures of epithelial and stromal cells isolated from normal adult rabbit cornea, when cocultured in the presence of cytochalasin B, produced latent collagenase, whereas neither cell type alone, nor the mixture in the absence of this agent, did so.
CellTetradecanoyl phorbol acetate stimulates latent collagenase production by cultured human endothelial cells
116 Citations1980David Moscatelli
TPA causes an increase in the production of latent collagenase by cultured human endothelial cells, which is a typical vertebrate collagenase as judged by the following criteria: it cleaved collagen into only two fragments which were three quarters and one quarter of the length of the intact molecule.
PubMedEnhanced collagenase production by fibroblasts derived from human basal cell carcinomas.
94 Citations1979Eugene A. Bauer, Jouni Uitto +2 more
PubMedEffect of collagenolytic activity in basal cell epithelioma of the skin on reconstituted collagen and physical properties and kinetics of the crude enzyme.
91 Citations1972Yuji Yamanishi, Mustafa Kh. Dabbous +1 more
Viscometric studies demonstrated that basal cell epithelioma crude enzyme decreased the specific viscosity of acid-soluble calf skin tropocollagen, and the ordered structure of the triple helical macromolecule was not affected during this treatment.
Proceedings of the National Academy of SciencesRegulation of stromal cell collagenase production in adult rabbit cornea: in vitro stimulation and inhibition by epithelial cell products.
86 Citations1980Barbara Johnson‐Wint
The data suggest that epithelial cells, in ways depending on their density, may modulate collagen degradation in the integument.
Biochemical and Biophysical Research CommunicationsIdentification of a type V collagenolytic enzyme
86 Citations1981Lance A. Liotta, William L. Lanzer +1 more
A neutral metal protease has been identified which cleaves native type V collagen under conditions where pepsinized type IV collagen or the interstitial collagens are not significantly degraded.
Current ScienceAnnual Reviews of Biochemistry, 2019
62 Citations2020Prasanna K. Santhekadur
Every year, the Annual Review of Biochemistry contains many important review articles on advanced aspects of modern biochemistry and serves as an important source of classical and canonical knowledge.
PubMedCollagenase and neutral protease activities in cultures of rabbit VX-2 carcinoma.
39 Citations1977Mustafa Kh. Dabbous, Audrey N. Roberts +1 more
Primary cultures of minced rabbit VX-2 carcinoma tissue were shown to release collagenolytic activity in serum-free medium, which appeared to be a multienzyme system composed of specific collagenase and neutral protease.
PubMedNeutron-induced mammary neoplasms in the rat.
22 Citations1972Howard H. Vogel, Robert Zaldívar
Fission neutrons are effective in producing mammary neoplasms in the female Sprague-Dawley rat, and the relative biological effectiveness (X/n) for this system appears to lie between 20 and 60, an extremely high value for any mammal.
Experimental and Molecular PathologySeparation of VX-2 rabbit carcinoma-derived cells capable of releasing collagenase
16 Citations1983Mustafa Kh. Dabbous, Mahmoud Eltorky +3 more
PathologyCollagenolytic activity by malignant tumours
15 Citations1982Robert L. O’Grady, P. J. Harrop +1 more
Five carcinomas and 5 sarcomas were investigated in relation to their production of neutral proteases capable of digesting polymeric collagen and these tumours possess collagenolytic ability in vitro which may be partly responsible for their invasive nature in vivo.
Molecular and Cellular BiochemistryChanges in the collagenolytic activity released by primary VX-2 carcinoma cultures as a function of tumor growth
10 Citations1977Mustafa Kh. Dabbous, Camelia Sobhy +2 more
Serum-free media of minced tissue cultures of VX-2 rabbit carcinoma contained a specific collagenolytic activity capable of releasing soluble radioactive peptides from [14C]-labeled collagen fibrils that was capable of reducing the viscosity of acid-soluble collagen solutions.
Biochemical and Biophysical Research CommunicationsThe action of tetranitromethane on acid-soluble tropocollagen
7 Citations1972Mustafa Kh. Dabbous, Mohamed Seif +1 more
Treatment of dilute solutions of acid-soluble calfskin collagen with tetranitromethane at pH 8.0 resulted in the nitration of all the tyrosine residues in tropocollagen without precipitation of the modified protein, indicating that some of the tyosine residues were involved in a reaction which did not produce 3-nitrotyrosine.
Archives of Oral BiologyStimulation of bone collagenase synthesis by mouse fibrosarcoma in resorbing bone in vitro culture
6 Citations1979Akihiro Matsumoto, S. Sakamoto +1 more
Fragments of transplantable mouse fibrosarcoma, co-cultured with 5-day-old mouse calvaria, stimulated bone to synthesize and/or release collagenase into the culture medium, whereas the tumour alone neither released nor synthesized collagenase.
Cancer Genetics and CytogeneticsCytogenetic analysis of collagenase-releasing rabbit VX-2 carcinoma-derived cells
5 Citations1983Mustafa Kh. Dabbous, Lena Haney +2 more
Cytogenetic studies showed that the E cells of rabbit VX-2 carcinoma were characterized by numerical and structural chromosomal changes which remained constant subsequent to serial in vitro culture and in vivo transplants in rabbits.
Annals of Otology Rhinology & LaryngologyCollagenase Activity in Squamous Cell Carcinoma of the Human Parotid Gland
5 Citations1982Cheng‐Chun Huang, Andrew Blitzer +1 more
Analysis of specimens of various human parotid gland tumors revealed that the levels of collagenase activity were higher in squamous cell carcinoma than in nonsquamous tumors, which suggests that collagenase may promote local connective tissue destruction associated with tumor invasion and lymph node metastasis.