Sensitivity of fibroblasts derived from ataxia-telangiectasia patients to calicheamicin γ1I
Mutation Research LettersPublished 1 November 1990
Neil F. Sullivan, Linden Lyne
Citations23
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TL;DR
Treatment with calicheamicin gamma 1I reveals SV40-transformed fibroblasts derived from ataxia telangiectasia (AT) patients to be 6-fold more sensitive to this drug when compared to controls.
Abstract
We report the hypersensitivity of SV40-transformed fibroblasts derived from ataxia telangiectasia (AT) patients to calicheamicin gamma 1I. In common with other free-radical generating agents such as bleomycin and ionizing radiation, treatment with calicheamicin gamma 1I reveals AT derived lines to be 6-fold more sensitive to this drug when compared to controls. Furthermore, in common with ionizing radiation, AT cells did not show dose-dependent inhibition of DNA synthesis after treatment with calicheamicin gamma 1I.
Keywords
Biochemistry, Genetics and Molecular Biology
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Cell survival experiments are reported which indicate that the clinically observed enhanced sensitivity of AT patients to ionising radiation is manifest at the cellular level.
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