Plasmid Detection and Sizing in Single Colony Lysates
SciencePublished 28 January 1977
Wayne M. Barnes
Citations187
SJR quartileQ1
SJR score10.42
SNIP6.62
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TL;DR
A simple and contained procedure for the rapid assay of the presence and size of plasmids similar to Col El is described.
Abstract
A simple and contained procedure for the rapid assay of the presence and size of plasmids similar to Col El is described. Bacteria are picked from an agar plate with a toothpick, lysed with dodecyl sulfate and heat, and placed directly on an agarose gel for electrophoresis.
Keywords
Biochemistry, Genetics and Molecular Biology
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