Randomly picked cosmid clones overlap the<i>pyr</i>B and<i>ori</i>C gap in the physical map of the<i>E.coli</i>chromosome
Nucleic Acids ResearchPublished 1 January 1988Open access
Vroni Knott, D. Jasper G. Rees, Zhi Cheng, G.G. Brownlee
Citations34
SJR quartileQ1
SJR score7.78
SNIP4.71
Generate an AI Snapshot to get a quick, structured summary of this paper.
Study Snapshot
ObjectiveStudy objective
MethodsResearch methodology
PopulationPopulation studied
Sample sizeSample sizes
OutcomesStudy outcomes here
ResultsStudy results comes here
LimitationsResearch study limitations comes here
A concise AI-generated summary of the paper will appear here once you click Generate AI Snapshot.
TL;DR
A new cloning strategy using sheared DNA, and a low copy, inducible cosmid vector were used in order to reduce bias in libraries, in conjunction with micro-methods for preparing cosmid DNA from a large number of clones.
Abstract
Sets of overlapping cosmid clones generated by random sampling and fingerprinting methods complement data at pyrB (96.5') and oriC (84') in the published physical map of E. coli. A new cloning strategy using sheared DNA, and a low copy, inducible cosmid vector were used in order to reduce bias in libraries, in conjunction with micro-methods for preparing cosmid DNA from a large number of clones. Our results are relevant to the design of the best approach to the physical mapping of large genomes.
Keywords
Biochemistry, Genetics and Molecular BiologyEnvironmental Science
CellSeparation of yeast chromosome-sized DNAs by pulsed field gradient gel electrophoresis
2,845 Citations1984David C. Schwartz, Charles R. Cantor
This pulsed field gradient gel electrophoresis fractionates intact S. cerevisiae chromosomal DNA, producing a molecular karyotype that greatly facilitates the assignment of genes to yeast chromosomes.
CellThe physical map of the whole E. coli chromosome: Application of a new strategy for rapid analysis and sorting of a large genomic library
1,763 Citations1987Yuji Kohara, Kiyotaka Akiyama +1 more
The strategy was to measure the sizes of partial restriction enzyme digests by hybridization with a vector probe in a manner analogous to nucleotide sequencing, and found that this strategy is applicable to analyses of the genomes of other organisms.
ScienceCloning of Large Segments of Exogenous DNA into Yeast by Means of Artificial Chromosome Vectors
1,514 Citations1987David Burke, Georges F. Carle +1 more
By offering a tenfold increase in the size of the DNA molecules that can be cloned into a microbial host, this system addresses a major gap in existing experimental methods for analyzing complex DNA sources.
Journal of Molecular BiologyHost specificity of DNA produced by Escherichia coli: Bacterial mutations affecting the restriction and modification of DNA
708 Citations1966William B. Wood
Behavior of the mutant strains in conjugation experiments is consistent with the view that bacterial DNA is restricted and modified by the same factors which act upon the DNA of phage λ, and that this restriction is responsible for the abnormally low frequency of recombinant formation observed in K12 × B crosses.
Proceedings of the National Academy of SciencesToward a physical map of the genome of the nematode <i>Caenorhabditis elegans</i>
630 Citations1986Alan Coulson, John Sulston +2 more
A technique for digital characterization and comparison of DNA fragments, using restriction enzymes, is described, being applied to fragments from the nematode Caenorhabditis elegans to facilitate cross-indexing of clones emanating from different laboratories and to construct a physical map of the genome.
Proceedings of the National Academy of SciencesRandom-clone strategy for genomic restriction mapping in yeast.
420 Citations1986Maynard V. Olson, James E. Dutchik +7 more
An approach to global restriction mapping is described that is applicable to any complex source DNA and shows that these data can be analyzed to produce regional maps of the yeast genome, extending in some cases for over 100 kilobases.
ScienceA Physical Map of the <i>Escherichia coli</i> K12 Genome
403 Citations1987Cassandra L. Smith, Jason G. Econome +3 more
A physical map of a genome is the structure of its DNA, and the resulting ordered set of fragments is a macrorestriction map that facilitates genetic and molecular studies on E. coli and serves as a model for further endeavors on larger genomes.
The EMBO JournalThe gene structure of human anti‐haemophilic factor IX.
367 Citations1984D S Anson, K.H. Andy Choo +5 more
The mRNA sequence of the human intrinsic clotting factor IX (Christmas factor) has been completed and is 2802 residues long, including a 29 residue long 5′ non‐coding and a 1390 residue long 3′ non-coding region, but excluding the poly(A) tail.
Nucleic Acids ResearchThe construction of cosmid libraries which can be used to transform eukaryotic cells
316 Citations1982F Grosveld, Torben Lund +4 more
Human and mouse cosmid libraries containing DNA inserts of about 40kb have been generated by an improved method and newly introduced genes are expressed accurately in L cells.
CellA large inverted duplication allows homologous recombination between chromosomes heterozygous for the proximal t complex inversion
230 Citations1987Bernhard G. Herrmann, Denise P. Barlow +1 more
The analyses show that the wild-type form of chromosome 17 contains an inverted duplication of an element of at least 650 kb that is present in only one copy in the t haplotype form.
GeneLow-copy-number plasmid-cloning vectors amplifiable by derepression of an inserted foreign promoter
198 Citations1984Jens Erik Løve Larsen, Kenn Gerdes +2 more
By insertion of a DNA fragment, containing the phage lambda pR promoter and the pM-promoted cI857 allele of the lambda repressor gene, in plasmid R1 upstream of the replication control genes, cloning vectors have been constructed which are present in one copy per chromosome at temperatures below 37 degrees C, and which display uncontrolled replication at 42 degrees C.
CellMolecular analysis of the Duchenne muscular dystrophy region using pulsed field gel electrophoresis
169 Citations1987Susan Kenwrick, Mark Patterson +3 more
The results demonstrate the applicability of PFGE for analysis of Xp21, and should facilitate the mapping of other translocations and deletions in this region, some of which lead to glycerol kinase deficiency and adrenal hypoplasia as well as DMD.
Biochemical JournalDNA sequence around the <i>Escherichia coli unc</i> operon. Completion of the sequence of a 17 kilobase segment containing <i>asnA, oriC, unc, glmS</i> and <i>phoS</i>
159 Citations1984John E. Walker, N Gay +2 more
The glmS gene encoding the amidotransferase, glucosamine synthetase, has been identified by homology with glutamine 5-phosphoribosylpyrophosphate amidotranferase.
The EMBO JournalA general method for preparing intact nuclear DNA.
91 Citations1984Peter R. Cook
Remarkably, considerable structure is preserved since the DNA is supercoiled and chromosomes retain their shape and are nevertheless accessible to enzymes and other probes.
Proceedings of the National Academy of SciencesA cosmid vector that facilitates restriction enzyme mapping.
55 Citations1985Peter Little, Sally H. Cross