Establishment of germ-line-competent embryonic stem (ES) cells using differentiation inhibiting activity
DevelopmentPublished 1 December 1990
Jennifer Nichols, E.P. Evans, Austin Smith
Citations331
SJR quartileQ1
SJR score1.72
SNIP0.98
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TL;DR
It is shown that ES cell lines can be derived and maintained in the absence of feeder layers using medium supplemented with purified DIA/LIF, which fulfils the essential function of feeders in the isolation of pluripotential stem cells.
Abstract
The regulatory factor Differentiation Inhibiting Activity/Leukaemia Inhibitory Factor (DIA/LIF) suppresses the differentiation of cultured embryonic stem (ES) cells. In the present study, it is shown that ES cell lines can be derived and maintained in the absence of feeder layers using medium supplemented with purified DIA/LIF. These cells can differentiate normally in vitro and in vivo and they retain the capacity for germ-line transmission. DIA/LIF therefore fulfils the essential function of feeders in the isolation of pluripotential stem cells.
Keywords
MedicineBiochemistry, Genetics and Molecular Biology
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