Proteolytic Enzymes Initiating Cell Division and Escape from Contact Inhibition of Growth

NaturePublished 1 July 1970

Max M. Burger

DOI10.1038/227170a0

Source URL

Citations511

SJR quartileQ1

SJR score18.29

SNIP10.16

TL;DR

It is suggested that the whole cell may respond to the proteolytic enzyme with initiation of new rounds of cell division and a concomitant escape from contact inhibition of growth, as is the case for malignant cells in tissue culture.

Abstract

BRIEF treatment with very low concentrations of proteolytic enzymes can bring about a change in the cell surface similar to that occurring in the chemical or viral transformation of normal to malignant cells1. This suggests to us that proteolytic enzymes may not only convert the surface structure into the type seen in malignant cells but that the whole cell may respond to the proteolytic enzyme with initiation of new rounds of cell division and a concomitant escape from contact inhibition of growth, as is the case for malignant cells in tissue culture2.

Keywords

Biochemistry, Genetics and Molecular Biology

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Several tissue culture cell lines that were transformed by a tumor virus have been found to react with an agglutinin, while under identical conditions their untransformed parent cell lines did notagglutinate.

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Results indicate that the surface membrane of transformed cells contains sites that interact with the alpha-MG binding sites of ConA, that such sites can be found on the surface membranes of normal cells after treatment with trypsin, and that the change in the surface structure from normal to transformed occurs in cells that are abortively transformed.

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3T3 cells grown in a medium containing a high concentration of serum failed to exhibit either density-dependent inhibition of growth or reduction in the transport of aminoisobutyric acid upon attainment of confluency, suggesting growth regulation apparently exemplified by density- dependent inhibition ofgrowth in vitro seems not to be due to general alterations in membrane permeability and transport.

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It is concluded that the enzymatic composition of tumor interstitial fluid is strongly shifted to favor the hydrolysis of polypeptides and most types of smaller l-forms of peptides and amides.

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All derivatives transformed by viruses agglutinated in similar conditions5, indicating that the ability of the tumour cell was acquired with viral transformation.

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24 Citations1968H. B. Rubin, Clarence Colby

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