A radioimmunoassay for corticosterone and its application to the measurement of stress in poultry
SteroidsPublished 1 December 1976
R. J. Etches
Citations189
SJR quartileQ2
SJR score0.62
SNIP0.69
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TL;DR
Tests of adrenal function showed that stimulation of the adrenal with exogenous ACTH and with dexamethasone caused an increase and decrease, respectively, in plasma concentrations of corticosterone.
Abstract
A radioimmunoassay for corticosterone was developed using an antibody to corticosterone-21-hemisuccinate:bovine serum albumin. The assay possessed good specificity, sensitivity and reproducibility and required minimal sample preparation. Tests of adrenal function showed that stimulation of the adrenal with exogenous ACTH and with dexamethasone caused an increase and decrease, respectively, in plasma concentrations of corticosterone. Exposure to cold environmental temperatures caused an increase in plasma corticosterone. Handling and the removal of blood samples by venepuncture had no effect upon the concentration of corticosterone. It was concluded that this assay would accurately measure the response to stresses which affect the pituitary-adrenal axis.
Keywords
ChemistryAgricultural and Biological SciencesBiochemistry, Genetics and Molecular Biology
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