Detection of mucus glycoconjugates in human conjunctiva by using the lectin‐colloidal gold technique in TEM
Acta OphthalmologicaPublished 1 August 1986
Piera Versura, Maria Cristina Maltarello, Mauro Cellini, R. Caramazza, R Laschi
Citations32
SJR quartileQ1
SJR score1.39
SNIP1.53
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TL;DR
The mucus glycoconjugates produced by conjunctival goblet cells in dry‐eye patients were studied by a specific cytochemical reaction in Transmission Electron Microscopy and it is thought these data may be useful in the search for a therapeutic mucomimetic drug.
Abstract
The mucus glycoconjugates produced by conjunctival goblet cells in dry-eye patients were studied by a specific cytochemical reaction in Transmission Electron Microscopy (TEM). Four lectins, proteins of vegetal origin which specifically bind glycosidic residues, (WGA, PNA, SBA and ConA) were used conjugated with colloidal gold as ultrastructural marker. We performed a quantitative analysis by counting the colloidal gold particles present on mucus granules. The results were compared with normal conditions. We found a decrease in sialic acid, N-acetyl-glucosamine, N-acetyl-galactosamine and galactose-N-acetyl-galactosamine and an increase in mannose. The different content of glycoconjugates in goblet cells may reflect in the change of physical and functional properties of mucus. We think these data may be useful in the search for a therapeutic mucomimetic drug.
Keywords
MedicineBiochemistry, Genetics and Molecular BiologyPharmacology, Toxicology and Pharmaceutics
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