Cytokinin Biosynthesis in Mutants of the Moss <i>Physcomitrella patens</i>
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TL;DR
Three cytokinin-over-producing mutants of the moss, Physcomitrella patens, have been shown to convert [8-(14)C]adenine to N(6)-[( 14)C](Delta(2)-isopentenyl)adenine, the presence of which was confirmed by thin layer Chromatography, high performance liquid chromatography, and recrystallization to constant specific radioactivity.
Abstract
Three cytokinin-over-producing mutants of the moss, Physcomitrella patens, have been shown to convert [8-(14)C]adenine to N(6)-[(14)C](Delta(2)-isopentenyl)adenine, the presence of which was confirmed by thin layer chromatography, high performance liquid chromatography, and recrystallization to constant specific radioactivity. The labeled cytokinin was detected in the culture medium within 6 hours and the tissue itself appears to contain both labeled N(6)-(Delta(2)-isopentenyl)adenine and N(6)-(Delta(2)-isopentenyl)adenosine monophosphate.