The Olfactory Neuron and the Blood-Brain Barrier
Novartis Foundation symposiumPublished 27 May 2008
A. J. Darin de Lorenzo
Citations146
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Abstract
This chapter contains section titled: Materials and Methods Observations Summary Discussion References
Keywords
NeuroscienceBiochemistry, Genetics and Molecular Biology
The Journal of Cell BiologyOLFACTORY CILIA IN THE FROG
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Olfactory epithelium from the frog was examined in the living state by light microscopy and in the fixed state by electron microscopy to support the theory that they are the locus where electrical excitation in the olfactory organ is initiated by contact with odorous substances.
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Observations of the olfactory mucosa with the electron microscope are discussed with respect to recent electrophysiological studies and the dendrite and axon of the bipolar cell are discussed.
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Evidence is presented indicating that the characteristically helical wall of the microtubules is made up of spherical subunits about 50 Å in diameter, with about 8 subunits in one turn of the helix, under certain conditions, the helical structure may be altered to form a wall comprised of longitudinal filaments.
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This chapter reviews some of the recent work that has revealed the important influence of olfaction on feeding, mating and reproduction, and movement orientation in the vertebrates.
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Microtubules from neurons are preserved in homogenates of mammalian brain by medium containing organic solvents at acidic pH by means of negative staining and electron microscopy and the relative concentration of microtubules in suspensions can be assayed.
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Hexylene glycol buffered at pH 6 .4 will preserve microtubules from mammalian brain in homogenates and cold, autolysis, divalent cations, and mitotic spindle inhibitors did not alter appreciably the stability of the microtubule in vitro.
NatureSmell, Taste, and Allied Senses in the Vertebrates
6 Citations1923W. M. B.
The mechanism of the senses of smell and taste is apt to be unduly neglected, probably on account of the fact that in civilised man these senses do not play a large part in intellectual processes, but they bring before us some interesting problems as to the nature of receptor organs in general.
