Identification, purification, and biological characterization of hematopoietic stem cell factor from buffalo rat liver-conditioned medium
CellPublished 1 October 1990
Krisztina M. Zsebo, Jette Wypych, Ian McNiece, Hsieng S. Lu, Kent A. Smith, Subash B. Karkare
Citations823
SJR quartileQ1
SJR score22.61
SNIP7.62
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TL;DR
A novel growth factor, stem cell factor (SCF), for primitive hematopoietic progenitors based on its activity on bone marrow cells derived from mice treated with 5-fluorouracil is identified and sequences of partial SCF cDNAs are presented.
Abstract
We have identified a novel growth factor, stem cell factor (SCF), for primitive hematopoietic progenitors based on its activity on bone marrow cells derived from mice treated with 5-fluorouracil. The protein was isolated from the medium conditioned by Buffalo rat liver cells. It is heavily glycosylated, with both N-linked and O-linked carbohydrate. Amino acid sequence following removal of N-terminal pyroglutamate is presented. The protein has potent synergistic activities in semisolid bone marrow cultures in conjunction with colony-stimulating factors. It is also a growth factor for mast cells. In two companion papers, we present the sequences of partial SCF cDNAs, identify SCF as a c-kit ligand, and map the SCF gene to the Sl locus of the mouse.
Keywords
MedicineBiochemistry, Genetics and Molecular Biology
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