Primordial Germ Cell Migration
Published 1 January 1986
Chris Wylie, David I. Stott, Peter J. Donovan
Citations34
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TL;DR
This study has revealed that germ cells link up with each other forming extensive networks during migration, and labels germ cells in whole-mount preparations with an antibody to the germ cell marker SSEA-1 reveal the spatial distribution of Germ cells in situ using confocal microscopy.
Abstract
Many, if not all, cells in the body can show motile activity when placed in a permissive environment. Cells held in an apparently immutable array in the intact body will nonetheless exhibit some sort of motile behavior when disaggregated and placed in culture. It is clear, therefore, that all cells possess or can make the machinery for cell movement. However, few cells actively move in the adult body; the phase of the life cycle during which large scale cell and tissue movements take place is during embryonic development. These movements result in the correct tissue architecture of the embryonic body becoming established.
Keywords
Biochemistry, Genetics and Molecular Biology
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A monoclonal antibody derived by fusion of mouse myeloma cells with spleen cells from a mouse immunized with F9 teratocarcinoma cells is described, which defines an embryonic stage-specific antigen.
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This chapter discusses germ plasm and the differentiation of the germ cell line and the histochemical evidence for the presence of RNA in polar granules of insects and germinal plasm of amphibians indicates that RNA is present only in eggs and early embryos, while a protein component is present throughout the germ cycle.
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The results suggest that P-granule segregation does not require either the spindle or cytoplasmic microtubules, but that this process as well as generation of other asymmetries does require cytoskeletal functions that depend on microfilaments.
NatureHaptotaxis and the Mechanism of Cell Motility
518 Citations1967S. B. CARTER
A hypothesis for the basis of tissue cell motility involving considerations of thermodynamics is put forward and Dr. Moilliet shows that the hypothesis is thermodynamically feasible.
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The results demonstrate the feasibility of identifying stage-specific antigenic determinants with monoclonal antibody prepared against embryos and the need for thorough screening on a variety of cell types to establish developmentally important cross-reactivities is emphasized.
The Journal of Cell BiologyChemotaxis by polymorphonuclear leukocytes
258 Citations1978S H Zigmond
The ability of a cell or organism to direct its movement along a chemical gradient has fascinated biologists for over 100 yr, but since the chemical nature of the gradient in most developmental systems is not known, it is difficult to study the mechanisms involved in the establishment of cell polarities.
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It is reported here that a fraction containing all the granulocytic/monocytic and erythroid progenitor cells, as well as cells which may be lymphoid progenitors, can be isolated from bone marrow using a combination of monoclonal antibodies.
Experimental Cell ResearchIn vitro culture of mouse primordial germ cells*1
121 Citations1983Massimo De Felici
During the culture period the proportion of female germ cells in meiosis increased and later stages of meiotic prophase were seen, and the gonadal environment is not essential for the progress of meiosis.
The Journal of ImmunologyNeutral glycolipid antigens as developmental markers of mouse teratocarcinoma and early embryos: an immunologic and chemical analysis.
102 Citations1982Keith R. Willison, Robin A. Karol +3 more
Interestingly, the 2 Forssman antibody-reactive glycolipids of F9 ECC differ in their mobility on TLC plates from the classical Forss man antigen extracted from sheep red blood cells, illustrating the potential problems in extrapolating from the coincident binding properties of an anti-glycolipid antibody to the chemical structure or abundance of an antigen in different cell types.
American ZoologistThe Germ Plasm of<i>Drosophila</i>: An Experimental System for the Analysis of Determination
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The posterior polar plasm of late stage oocytes of Drosophila melanogaster has been shown to be functional in inducing germ cells, thus demonstrating that the active components are formed during oogenesis.
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It is suggested that p.
Experimental Cell ResearchEvidence for substrate guidance of primordial germ cells
31 Citations1979Christopher Wylie, Janet Heasman +2 more
Using the scanning electron microscope, it is demonstrated that the coelpmic lining cells, beneath which PGCs migrate up the dorsal mesentery of the gut, are orientated in the direction of travel, raising the intriguing possibility that P GCs are guided for at least part of their migration in Xenopus laevis embryos by a substrate-guidance mechanism.
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