A Serum Shock Induces Circadian Gene Expression in Mammalian Tissue Culture Cells
CellPublished 1 June 1998Open access
Aurélio Balsalobre, Francesca Damiola, Ueli Schibler
Citations2,001
SJR quartileQ1
SJR score22.61
SNIP7.62
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TL;DR
The treatment of cultured rat-1 fibroblasts or H35 hepatoma cells with high concentrations of serum induces the circadian expression of various genes whose transcription also oscillates in living animals, and thus mimics light-induced immediate-early gene expression in the suprachiasmatic nucleus.
Abstract
The treatment of cultured rat-1 fibroblasts or H35 hepatoma cells with high concentrations of serum induces the circadian expression of various genes whose transcription also oscillates in living animals. Oscillating genes include rper1 and rper2 (rat homologs of the Drosophila clock gene period), and the genes encoding the transcription factors Rev-Erb alpha, DBP, and TEF. In rat-1 fibroblasts, up to three consecutive daily oscillations with an average period length of 22.5 hr could be recorded. The temporal sequence of the various mRNA accumulation cycles is the same in cultured cells and in vivo. The serum shock of rat-1 fibroblasts also results in a transient stimulation of c-fos and rper expression and thus mimics light-induced immediate-early gene expression in the suprachiasmatic nucleus.
Keywords
MedicineNeuroscienceBiochemistry, Genetics and Molecular Biology
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