Translation of cyclin mRNA is necessary for extracts of activated Xenopus eggs to enter mitosis
CellPublished 1 March 1989
Jeremy Minshull, J. Julian Blow, Tim Hunt
Citations437
SJR quartileQ1
SJR score22.61
SNIP7.62
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TL;DR
It is shown that Xenopus oocytes contain mRNAs encoding two cyclins that are major translation products in a cell-free extract from activated eggs, and that the synthesis of these cyclins is necessary for mitotic cell cycles in cleaving Xenopus embryos.
Abstract
The cyclins are a family of proteins encoded by maternal mRNA. Cyclin polypeptides accumulate during interphase and are destroyed during mitosis at about the time of entry into anaphase. We show here that Xenopus oocytes contain mRNAs encoding two cyclins that are major translation products in a cell-free extract from activated eggs. Cutting these mRNAs with antisense oligonucleotides and endogenous RNAase H blocks entry into mitosis in a cell-free egg extract. The extracts can enter mitosis if either of the cyclin mRNAs is left intact. We conclude that the synthesis of these cyclins is necessary for mitotic cell cycles in cleaving Xenopus embryos.
Keywords
MedicineBiochemistry, Genetics and Molecular Biology
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