Multiple sequence-specific transcription factors modulate cytomegalovirus enhancer activity in vitro.
Molecular and Cellular BiologyPublished 1 April 1988Open access
Peter Ghazal, Henryk Luboń, Lothar Hennighausen
Citations51
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Abstract
The possibility of DNA-binding proteins interacting in vitro with the polymerase II transcriptional machinery was explored by using a competition assay with individual target sequences for enhancer-binding factors. Transcription factors binding to at least five specific enhancer sequences mediate the activity of the human cytomegalovirus immediate-early 1 gene in vitro. Furthermore, our data suggest that individual DNA-bound enhancer factors can interact with the promoter transcription complex.
Keywords
MedicineBiochemistry, Genetics and Molecular Biology
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