Regulation of sterol transport in human microvascular endothelial cells.

In cultured human dermal microvessel endothelial cells, the rate of efflux was coupled to an equivalent high rate of sterol net transport from the cells to the medium, and this net transport was linked with esterification via lecithin:cholesterol acyltransferase.

Abstract

In cultured human dermal microvessel endothelial cells, the rate of efflux (about twofold greater than for fibroblasts under equivalent conditions) was coupled to an equivalent high rate of sterol net transport from the cells to the medium. This net transport was linked with esterification via lecithin:cholesterol acyltransferase. Since the use of free sterol by plasma transferase is constant, such increased net transport indicates that endothelial cells are highly efficient, in competition with plasma lipoproteins, in supplying free sterol for esterification. These results indicate the marked ability of endothelial cells to regulate and maintain their sterol balance in the face of high sterol levels to which these cells are uniquely exposed in human plasma.