Codon usage can affect efficiency of translation of genes in<i>Escherichia coli</i>
Nucleic Acids ResearchPublished 1 January 1984Open access
M K Robinson, R.J. Lilley, Stephen F. Little, J.S. Emtage, Geoffrey Yarranton, P. Stephens
Citations296
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TL;DR
By inserting synthetic oligonucleotides into a highly expressed gene in E. coli it has been shown that unfavourable codon usage can reduce the maximum translation rate of a protein.
Abstract
By inserting synthetic oligonucleotides into a highly expressed gene in E. coli it has been shown that unfavourable codon usage can reduce the maximum translation rate of a protein. However, in the case of the codon used (AGG), a significant effect on translation was only seen at very high transcription rates from a gene containing multiple copies of the unfavourable codon.
Keywords
Biochemistry, Genetics and Molecular Biology
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