Prospects for drug screening using the reverse two-hybrid system
Trends in biotechnologyPublished 1 September 1999
Marc Vidal, Hideki Endoh
Citations83
SJR quartileQ1
SJR score2.36
SNIP2.27
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TL;DR
The reverse two-hybrid system appears to have several properties that would be critical for the success of this approach, and may represent optimal targets in the chemical-genomics era.
Abstract
Rational drug-screening strategies have been limited by the number of available protein targets. The fields of genomics and functional genomics are now merging into 'chemical genomics' approaches, in which large numbers of potential target proteins can be used in standardized high-throughput drug-screening assays. Because protein-protein interactions are critical to most biological processes and can be tested in standardized assays, they may represent optimal targets in the chemical-genomics era. The reverse two-hybrid system appears to have several properties that would be critical for the success of this approach.
Keywords
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