Sequence of a mouse germ-line gene for a variable region of an immunoglobulin light chain.
Proceedings of the National Academy of SciencesPublished 1 March 1978Open access
Susumu Tonegawa, Allan M. Maxam, Richard Tizard, Olivier Bernard, W Gilbert
Citations417
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TL;DR
The sequence confirms that the variable region gene lies on the DNA separated from the constant region of a mouse immunoglobulin light chain, the VlambdaII gene, andHypervariable region codons appear in the germ-line sequence.
Abstract
We have determined the sequence of the DNA of a germ-line gene for the variable region of a mouse immunoglobulin light chain, the VlambdaII gene. The sequence confirms that the variable region gene lies on the DNA separated from the constant region. Hypervariable region codons appear in the germ-line sequence. A sequence for the hydrophobic leader, 19 amino acids that are cleaved from the amino terminus of the protein, appears near, but not continuous with, the light chain structural sequence: most of the leader sequence is separated from the rest of the gene by 93 bases of untranslated DNA.
Keywords
Biochemistry, Genetics and Molecular Biology
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