Biochemical and Genetic Studies of Recombination Proficiency in <i>Escherichia coli</i> , I. Enzymatic Activity Associated with <i>recB</i> <sup>+</sup> and <i>recC</i> <sup>+</sup> Genes
Proceedings of the National Academy of SciencesPublished 1 April 1970Open access
Stephen D. Barbour, Alvin J. Clark
Citations197
Generate an AI Snapshot to get a quick, structured summary of this paper.
Study Snapshot
ObjectiveStudy objective
MethodsResearch methodology
PopulationPopulation studied
Sample sizeSample sizes
OutcomesStudy outcomes here
ResultsStudy results comes here
LimitationsResearch study limitations comes here
A concise AI-generated summary of the paper will appear here once you click Generate AI Snapshot.
TL;DR
An adenosine triphosphate-dependent deoxyribonuclease activity has been detected in lysates of recB(+)recC(+) strains, and mutations in recB or recC lead to loss of this activity, suggesting that these two genes determine the nucleaseActivity.
Abstract
An adenosine triphosphate-dependent deoxyribonuclease activity has been detected in lysates of recB(+)recC(+) strains. Mutations in recB or recC lead to loss of this activity, suggesting that these two genes determine the nuclease activity. The over-all reaction in crude lysates digests native DNA to nucleoside monophosphates. Complementation between recB21 and recC22 in vivo leads to normal levels of ATP-dependent nuclease activity. No complementation in vitro has been detected. Mutations in a third recombination gene (recA) do not alter significantly the wild-type levels of this nuclease activity.
Keywords
Biochemistry, Genetics and Molecular Biology
Proceedings of the National Academy of SciencesFormation of merodiploids in matings with a class of Rec- recipient strains of Escherichia coli K12.
448 Citations1968Brooks Low
Bacteriological ReviewsRevised linkage map of Escherichia coli
423 Citations1967Austin L. Taylor, C D Trotter
A large number of Gene Loci have been identified that have shown the ability to transfer from E.coli to E. Coli through contact chemoreception, a process known as “correlation of Cotransduction Frequency with Map Distance in Minutes”.
Journal of Biological ChemistryThe Deoxyribonucleases of Escherichia coli
403 Citations1960I Lehman
There are a number of enzymes present in cell-free extracts of Escherichia coli which can degrade deoxyribonucleic acid (DNA) smaller polynucleotides from it these.
Journal of BacteriologyGenetic Location of Certain Mutations Conferring Recombination Deficiency in <i>Escherichia coli</i>
342 Citations1969Neil Willetts, Alvin J. Clark +1 more
Four mutations conferring recombination deficiency have been cotransduced with cysC and pheA and, despite the different degrees of apparent dominance, all may lie in the recA gene.
Journal of BacteriologyCharacteristics of Some Multiply Recombination-Deficient Strains of <i>Escherichia coli</i>
268 Citations1969Neil Willetts, Alvin J. Clark
It is concluded that in a Rec(+) strain, the recA(+) product acts to inhibit DNA breakdown determined by the recC(+) and recB(+) products.
Proceedings of the National Academy of SciencesFormation of covalent circles of lambda DNA by E. coli extracts.
232 Citations1967Martin Gellert
Journal of Molecular BiologyEndonuclease I-deficient and ribonuclease I-deficient Escherichia coli mutants
210 Citations1968Hildegard Dürwald, Hartmut Hoffmann‐Berling
A method has been developed to select for Escherichia coli mutants which lack certain nucleases, and mutants deficient in endonuclease I or RNase I were essentially identical to wild-type with respect to their general biological properties.
Biochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein SynthesisLysis of Escherichia coli with a neutral detergent
207 Citations1967G. Nigel Godson, Robert L. Sinsheimer
All strains of E. coli tested were susceptible to lysis by the neutral detergent Brij-58 and the cellular DNA, after lysis, may be almost completely sedimented by centrifugation at 3000 × g for 5 min.
Journal of Molecular BiologyAbnormal metabolic response to ultraviolet light of a recombination deficient mutant of Escherichia coli K12
196 Citations1966Alvin J. Clark, Michael J. Chamberlin +2 more
A numerical argument is offered to establish the ability of Rec − cells successfully to replace excised pyrimidine-dimer-containing regions of the DNA with normal nucleotide residues.
Journal of BacteriologyGenetic Analysis of Recombination-Deficient Mutants of <i>Escherichia coli</i> K-12 Carrying <i>rec</i> Mutations Cotransducible with <i>thyA</i>
165 Citations1969Neil Willetts, David W. Mount
The rec mutations carried by 20 strains of Escherichia coli K-12 which are defective in genetic recombination and sensitive to ultraviolet light and X rays, and whose lambda lysogens show spontaneous phage production, have been mapped near thyA.
Cold Spring Harbor Symposia on Quantitative BiologyEnzymatic DNA Degradation in E. coli: Its Relationship to Synthetic Processes at the Chromosome Level
160 Citations1968G. Buttin, Mark D. Wright
Journal of Cellular PhysiologyThe beginning of a genetic analysis of recombination proficiency
158 Citations1967Alvin J. Clark
Recombination‐deficient mutants of Escherichia coli have been isolated as members of a class showing reduced ability to mother recombinants when crossed with a given Hfr strain, and a continuous ranking of fertilities is observed.
Proceedings of the National Academy of SciencesThe enzymatic repair of DNA. I. Formation of circular lambda-DNA.
131 Citations1967M L Gefter, A Becker +1 more
Cold Spring Harbor Symposia on Quantitative BiologyThe General Recombination System of Bacteriophage
40 Citations1968Ethan Signer, Harrison Echols +5 more