Regulation of human interleukin-2 gene: Functional DNA sequences in the 5′ flanking region for the gene expression in activated T lymphocytes
CellPublished 1 August 1986
Takashi Fujita, Hiroshi Shibuyà, Tetsuo Ohashi, K Yamanishi, Tadatsugu Taniguchi
Citations288
SJR quartileQ1
SJR score22.61
SNIP7.62
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TL;DR
The presence of unique DNA sequences in the 5' flanking region of the human IL-2 gene that control induced T-cell-specific gene expression are demonstrated and function in an orientation-independent manner and activate a heterologous promoter which is otherwise inert in induced T cells.
Abstract
Interleukin-2 (IL-2) is a lymphokine that plays a crucial role in the immune system, especially in the growth control of T lymphocytes. Expression of this lymphokine is restricted to activated T lymphocytes. Here we demonstrate the presence of unique DNA sequences in the 5' flanking region of the human IL-2 gene that control induced T-cell-specific gene expression. We also show that the DNA sequences function in an orientation-independent manner and activate a heterologous promoter which is otherwise inert in induced T cells. The DNA, which spans about 200 bp, contains regions with sequence homology to LTR sequences of HTLV-III (or LAV) and the 5' upstream region of the IL-2 receptor and interferon-gamma genes.
Keywords
Immunology and MicrobiologyBiochemistry, Genetics and Molecular Biology
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