cDNA Cloning and Expression of theXenopus laevisVitellogenin Receptor

Although Northern blotting analysis revealed that this gene was expressed as a major transcript in Xenopus ovary, weak but significant expression was observed in other tissues by RT-PCR analysis, the fact that major expression of the gene occurs in the ovary suggests that it has an important function in this organ.

Abstract

A Xenopus laevis oocyte cDNA library was screened with a PCR-generated X. laevis vitellogenin (VTG) receptor probe and a 3.6 kb cDNA clone containing the entire open reading frame, and 5' and 3' noncoding regions were isolated. The deduced amino acid sequence was 72% homologous to the chicken VLDL/VTG receptor, and the characteristic domains were highly conserved. Ligand binding analysis confirmed that the cloned receptor was Xenopus VTG-specific. Although Northern blotting analysis revealed that this gene was expressed as a major transcript of 3.6 kb in Xenopus ovary, weak but significant expression was observed in other tissues by RT-PCR analysis. The fact that major expression of the gene occurs in the ovary suggests that it has an important function in this organ.