Role of coliphage M13 gene 5 in single-stranded DNA production
Journal of Molecular BiologyPublished 1 November 1971
John S. Salstrom, David Pratt
Citations147
SJR quartileQ1
SJR score2.21
SNIP1.13
Generate an AI Snapshot to get a quick, structured summary of this paper.
Study Snapshot
ObjectiveStudy objective
MethodsResearch methodology
PopulationPopulation studied
Sample sizeSample sizes
OutcomesStudy outcomes here
ResultsStudy results comes here
LimitationsResearch study limitations comes here
A concise AI-generated summary of the paper will appear here once you click Generate AI Snapshot.
TL;DR
The phage M13 gene 5 protein is shown to play a negative role in the production of viral single-stranded DNA.
Abstract
The phage M13 gene 5 protein is shown to play a negative role in the production of viral single-stranded DNA. This protein blocks the function of a DNA polymerase which is capable of converting intracellular single strands to double-stranded replicative form molecules.
Keywords
Immunology and MicrobiologyMedicineEnvironmental Science
Analytical BiochemistryA simple efficient liquid scintillator for counting aqueous solutions in a liquid scintillation counter
7,507 Citations1960George A. Bray
A modification of the naphthalene-dioxane-PPO liquid scintillator has been described which will allow up to 3.0 ml of an aqueous solution to be counted, which has been used to count carbon-14 and tritium in urine, plasma, and liver homogenate.
Journal of Molecular BiologyGenetic control of bacteriophage M13 DNA synthesis
147 Citations1968David Pratt, William S. Erdahl
The experiments show that no viral protein synthesis is needed to permit conversion of the infecting DNA strand to an RF molecule, but that the product of M13 gene 2 is required for the build-up of the RF pool and theProduct of gene 5 for progeny single-strand production.
VirologyConditional lethal mutants of the small filamentous coliphage M13
147 Citations1966David Pratt, Helen Tzagoloff +1 more
Ninety-six conditional lethal mutants of coliphage M13 have been isolated and assigned to complementation groups as part of a program to determine the number of cistrons and their functions in this small DNA virus.
Journal of Molecular BiologyThe topology of DNA from the small filamentous bacteriophage fd
144 Citations1966D.A. Marvin, Heinz Schaller
Results are taken to indicate that the native DNA molecule is resistant to a variety of agents which destroy secondary structure and has a stable ring topology.
VirologyConditional lethal mutants of the small filamentous coliphage M13
139 Citations1969David Pratt, Helen Tzagoloff +1 more
These results, together with results published elsewhere, show that gene 3 codes for the phage attachment protein, probably present as a single subunit at one end of the filamentous virion, while gene 8 code for the major coat protein subunit.
Zeitschrift für Naturforschung BUntersuchungen über „kleine“ E. coli K 12 Bakteriophagen
115 Citations1963P. H. Hofschneider
The isolation and some properties of three small bacteriophages growing on E. coli K 12-strains will be described and infectious nucleic acid of all three phages can be prepared.
Zeitschrift für Naturforschung BEin fädiger DNS-Phage (fd) und ein sphärischer RNS-Phage (fr), wirtsspezifisch für männliche Stämme von E. coli
97 Citations1963Hartmut Hoffmann‐Berling, D.A. Marvin +1 more
Unpurified preparations of the phages fd and fr contain particles —probably incomplete phages — which are made up of the specific phage coat protein and a reduced proportion of nucleic acid.
Journal of Molecular BiologyReplication of bacteriophage M13
87 Citations1969Dan S. Ray
Sucrose-gradient sedimentation of DNA from cells pulse-labeled with tritiated thymidine at 130 minutes after M13 infection shows a flow of material from RF ‡ to progeny single strands, which is found in both viral and complementary strands of RF only after long-term labeling from the time of infection.
Cellular and Molecular Life SciencesSampling of virus particles and macromolecules sedimented in an equilibrium density gradient
78 Citations1960Waclaw Szybalski
La distribution de composés macromoléculaires, stratifiés dans un gradient de densité de CsCl établi par une ultracentrifugation prolongée, est déterminée quantitativement par le recoupage du contenu du tube.
Biochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein SynthesisReplication of bacteriophage M13
74 Citations1969Dan S. Ray, Randy Schekman
Velocity sedimentation at high ionic strength, of DNA released from M13-infected bacteria lysed under gentle conditions, provides a simple method for separating phage-specific DNA components from bacterial DNA.
Journal of Molecular BiologyReplication of the single-stranded DNA of the male-specific bacteriophage M13
73 Citations1966Dan S. Ray, Hanns-P. Bscheider +1 more
The intracellular DNA has been isolated from Escherichia coli F+ cells infected with the small male-specific bacteriophage M13 and fractionated on a methylated albumin-kieselguhr column and was found to be resistant to thermal denaturation, suggesting that it may be a closed ring.
Journal of Molecular BiologyThe synthesis of messenger RNA without protein synthesis
69 Citations1964Jerry Stern, Masahiro Sekiguchi +2 more
Observations are consistent with the hypothesis that the RNA made in the absence of an essential amino acid and of protein synthesis is largely messenger RNA.
Journal of Molecular BiologyP1-like plasmid in Escherichia coli 15
50 Citations1970Hideo Ikeda, Manabu Inuzuka +1 more
In Escherichia coli 15, there are two plasmids of molecular lengths of 32.9 μ and 53.9μ, in addition to the previously discovered plasmid of molecular length of 0.96 μ, which confers a specific restriction and modification of DNA differing from that conferred by prophage P1 and is present to the extent of about one per bacterial chromosome.
Journal of Molecular BiologyFilamentous bacterial viruses
48 Citations1971Barbara Höhn, Helga Lechner +1 more
The results are discussed in terms of an inhibitor model for the switch from double-strand replication to single-Strand synthesis and the abrupt decrease in acceleration of net DNA synthesis corresponds in time to the onset of synthesis of single-stranded viral DNA.
Journal of Molecular BiologyThe eight genes of bacteriophages φX174 and S13 and comparison of the phage-specified proteins
36 Citations1970Ya‐Chung Jeng, David H. Gelfand +3 more
Complementation tests between phages S13 and φX174 show that the two phages have at least eight homologous essential genes.
Biochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein SynthesisPhysicochemical studies on the minicircular DNA in Escherichia coli 15
34 Citations1970Chong Sung Lee, Norman Davidson
Two presumed replicating species, the σ form and the θ form, of the minicircular DNA are observed in electron micrographs of cell lysates, andRenaturation kinetics measurements show that all of the approx.
Journal of Molecular BiologyReplication of bacteriophage M13
27 Citations1970Dan S. Ray
All of the DNA contained in the phage particles released following the removal of chloramphenicol is of the usual viral type, and up to one-third of the single strands produced are of the complementary type and are circular.