Carbohydrate metabolism and transport in Bacillus subtilis

Pleiotropic point mutations termed ctr, which affect carbon source utilization have been mapped on the B. subtilis chromosome by PBS1 mediated transduction, between the fru and metC markers, which are related to the absence of an Enzyme I like component in the phosphoenolpyruvate phosphotransferase system.

Abstract

Pleiotropic point mutations termed ctr, which affect carbon source utilization have been mapped on the B. subtilis chromosome by PBS1 mediated transduction, between the fru and metC markers. The ctr mutants are unable to carry on the uptake of radioactive fructose, glucose (méthyl-α-D-glucopyranoside), mannose, mannitol and sucrose, which is related to the absence of an Enzyme I like component in the phosphoenolpyruvate phosphotransferase system. The pleiotropic effect of the mutation includes the unability of the cells to grow on glycerol as carbon source, though glycerol transport is not mediated by the PEP phosphotransferase system.