A quantitative in vitro focus assay for bovine papilloma virus
VirologyPublished 1 June 1980
Israel Dvoretzky, Ralph Shober, Sisir K. Chattopadhyay, Douglas R. Lowy
Citations309
SJR quartileQ2
SJR score0.74
SNIP0.60
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TL;DR
A quantitative tissue culture assay for bovine papilloma virus (BPV) has been developed and focus formation followed single-hit kinetics, suggesting that a single particle was required for focus induction.
Abstract
A quantitative tissue culture assay for bovine papilloma virus (BPV) has been developed. Extracts of bovine papillomas which contained either BPV-1 or BPV-2 induced distinct foci in two mouse cell lines (NIH 3T3 and C127). Focus formation followed single-hit kinetics, suggesting that a single particle was required for focus induction. Preincubation of extracts with rabbit anti-BPV-1 serum neutralized their infectivity. The morphologically transformed cells contained BPV DNA sequences, grew in low serum and in soft agar, and were tumorigenic for nude mice.
Keywords
Agricultural and Biological SciencesMedicineBiochemistry, Genetics and Molecular Biology
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