Genetic map of Saccharomyces cerevisiae, edition 9
Microbiological ReviewsPublished 1 September 1985Open access
Robert Mortimer, David Schild
Citations251
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TL;DR
This chapter discusses new additions to Chromosome I, II, III, and IV, as well as descriptions of existing ones and some examples of new ones proposed.
Keywords
Biochemistry, Genetics and Molecular BiologyEngineering
CellSeparation of yeast chromosome-sized DNAs by pulsed field gradient gel electrophoresis
2,845 Citations1984David C. Schwartz, Charles R. Cantor
This pulsed field gradient gel electrophoresis fractionates intact S. cerevisiae chromosomal DNA, producing a molecular karyotype that greatly facilitates the assignment of genes to yeast chromosomes.
Nucleic Acids ResearchSeparation of chromosomal DNA molecules from yeast by orthogonal-field-alternation gel electrophoresis
884 Citations1984Georges F. Carle, Maynard V. Olson
The design of the apparatus, the electrophoretic protocol, and the sample-handling procedures that are employed are described, and several of which have been shown by DNA-DNA hybridization to hybridize uniquely to different chromosome-specific hybridization probes are described.
NatureIsolation of a yeast centromere and construction of functional small circular chromosomes
803 Citations1980Louise Clarke, John Carbon
The centromeric DNA (CEN3) from yeast chromosome III has been isolated on a 1.6 kilobase-pair segment of DNA located near the centromere-linked CDC10 locus of Saccharomyces cerevisiae.
GeneticsGENETIC CONTROL OF THE CELL DIVISION CYCLE IN YEAST: V. GENETIC ANALYSIS OF <i>cdc</i> MUTANTS
741 Citations1973Leland H. Hartwell, Robert Mortimer +2 more
The gene products that are defined by the cdc cistrons are essential for the completion of the cell cycle in haploids of a and alpha mating type and in a/alpha diploid cells and the same genes control thecell cycle in each of these stages of the life cycle.
Proceedings of the National Academy of SciencesDNA topoisomerase II mutant of Saccharomyces cerevisiae: topoisomerase II is required for segregation of daughter molecules at the termination of DNA replication.
685 Citations1984Stephen DiNardo, K Voelkel +1 more
The results suggest that DNA topoisomerase II is necessary for the segregation of chromosomes at the termination of DNA replication.
CellEvidence for transposition of dispersed repetitive DNA families in yeast
585 Citations1979John Cameron, Elwyn Loh +1 more
Mutation Research/Fundamental and Molecular Mechanisms of MutagenesisA genetic study of X-ray sensitive mutants in yeast
475 Citations1974John C. Game, Robert Mortimer
Examination of the survival responses of multiple-mutant strains indicated a minimum of two pathways in the repair of X-ray damage, and a number of the mutants have been mapped and these were found to be dispersed over the genome.
CellGenetic analysis of yeast RAS1 and RAS2 genes
463 Citations1984Tohru Kataoka, Scott Powers +5 more
It is determined that neither RAS1 nor RAS2 are by themselves essential genes, however, ras1 - ras2 - spores of doubly heterozygous diploids are incapable of resuming vegetative growth.
NatureA yeast gene encoding a protein homologous to the human c-has/bas proto-oncogene product
394 Citations1983Dieter Gallwitz, Cornelia Donath +1 more
The identification of a gene in S. cerevisiae which codes for a 206 amino acid protein (YP2) that exhibits striking homology to the p21 products of the human c-has/bas proto-oncogenes4,5 and the transforming p21 proteins of the Harvey (v-rasH)6 and Kirsten ( v-rasK)7 murine sarcoma viral oncogene.
Microbiological ReviewsGenetic map of Saccharomyces cerevisiae
391 Citations1980Robert Mortimer, David Schild
Proceedings of the National Academy of SciencesIsolation and characterization of yeast mutants deficient in adenylate cyclase and cAMP-dependent protein kinase.
352 Citations1982Kengo Matsumoto, Isao Uno +2 more
The results indicate that cAMP is an essential factor for yeast cells to proceed through the cell cycle via the activation of protein kinase.
GeneticsMUTATIONS AFFECTING TY-MEDIATED EXPRESSION OF THE <i>HIS4</i> GENE OF <i>SACCHAROMYCES CEREVISIAE</i>
350 Citations1984Fred Winston, Deborah T. Chaleff +2 more
Seven unlinked genes (SPT genes) that affect the phenotypes of Ty and delta insertion mutations in the 5' noncoding region of the HIS4 gene of S. cerevisiae are identified.
Molecular and Cellular BiologyIsolation and Genetic Analysis of <i>Saccharomyces cerevisiae</i> Mutants Supersensitive to G1 Arrest by a Factor and α Factor Pheromones
319 Citations1982Russell K. Chan, Carol A. Otte
Eight independently isolated mutants which are supersensitive (Sst-) to the G1 arrest induced by the tridecapeptide pheromone alpha factor were identified by screening mutagenized Saccharomyces cerevisiae MATa cells on solid medium for increased growth inhibition by alpha factor.
GeneticsCHROMOSOME MAPPING IN SACCHAROMYCES: CENTROMERE-LINKED GENES
299 Citations1960D C Hawthorne, Robert Mortimer
Proceedings of the National Academy of SciencesPositive regulation in the general amino acid control of Saccharomyces cerevisiae.
280 Citations1983A G Hinnebusch, G R Fink
Study of regulatory behavior of strains containing both aas and tra3 mutations and strains containing the cloned AAS genes in high copy number suggests that AAS1 and AAS2 are negative regulators of TRA3, which is a negative regulator of AAS3.
Journal of BacteriologyIsolation and Characterization of Acid Phosphatase Mutants in <i>Saccharomyces cerevisiae</i>
278 Citations1973Akio Toh‐e, Yoshinami Ueda +2 more
Since both repressible acid and alkaline phosphatases were affected simultaneously in the phoR, phoD, and phoS mutants, it was concluded that these enzymes were under the same regulatory mechanism or that they shared a common polypeptide.
Genetics<i>MAR1</i>—A REGULATOR OF THE <i>HM</i> <b>a</b> AND <i>HMα</i> LOCI IN <i>SACCHAROMYCES CEREVISIAE</i>
278 Citations1979Amar J. S. Klar, Seymour Fogel +1 more
The genetic evidence reported here is consistent with a central feature of the "cassette model", namely that HMalpha and hma carry MATa information and HMa and hmalpha carry MATalpha information.
GeneticsENZYMATIC EXPRESSION AND GENETIC LINKAGE OF GENES CONTROLLING GALACTOSE UTILIZATION IN SACCHAROMYCES
271 Citations1964H. C. Douglas, D C Hawthorne
Evidence will be presented to show that gu7 and gal" block the synthesis of transferase and epimerase, respectively, and that gu4 prevents the synthesisation of all three of the galactose genes.
Cold Spring Harbor Monograph ArchiveRegulation of Amino Acid and Nucleotide Biosynthesis in Yeast
266 Citations1982Elizabeth W. Jones, Gerald R. Fink
This paper attempts to summarize the current status of the knowledge of regulation of anabolic pathways, which includes pathways of biosynthesis of all 20 amino acids, of purine and pyrimidine nucleotides, and of single carbon metabolism.
NatureRequirement of either of a pair of ras-related genes of Saccharomyces cerevisiae for spore viability
229 Citations1984Kelly Tatchell, Deborah T. Chaleff +2 more
Cells of the yeast, Saccharomyces cerevisiae, containing disruptions of either of two genes that are members of the ras oncogene family are viable, but haploid yeast spores carrying disruptions of both genes fail to grow.
GeneticsREGULATION OF GENES CONTROLLING SYNTHESIS OF THE GALACTOSE PATHWAY ENZYMES IN YEAST
224 Citations1966H. C. Douglas, D C Hawthorne
The occurrence of mutations in the yeast galactose system which display the dominant constitutive phenotype and the relationship of these mutants to the GA, region and to the structural genes for the Galactose enzymes is the subject of the present paper.
GeneticsA SUPPRESSOR OF <i>snf1</i> MUTATIONS CAUSES CONSTITUTIVE HIGH-LEVEL INVERTASE SYNTHESIS IN YEAST
220 Citations1984Marian Carlson, Barbara C Osmond +2 more
Synthesis of secreted invertase in s sn6 strains was found to be constitutive, that is, insensitive to glucose repression; moreover, the ssn6 mutations also conferred constitutivity in a wild-type ( SNF1 ) genetic background and are, therefore, not merely suppressors of snf1 .
Advances in geneticsMap Construction in Neurospora Crassa
214 Citations1954R. W. Barratt, Dorothy Newmeyer +2 more
GeneticsGENETIC MAPPING IN SACCHAROMYCES IV. MAPPING OF TEMPERATURE-SENSITIVE GENES AND USE OF DISOMIC STRAINS IN LOCALIZING GENES
211 Citations1973Robert Mortimer, D C Hawthorne
Through use of tetrad, random spore, trisomic, and mitotic analysis procedures a large number of genes, including 48 new genetic markers, were studied for their locations on the genetic maps of the yeast Saccharomyces cerevisiae, finding Functionally-related sets of genes generally were found to be dispersed over the genome.
GeneticsGENETIC ANALYSIS OF MUTANTS OF <i>SACCHAROMYCES CEREVISIAE</i> RESISTANT TO THE HERBICIDE SULFOMETURON METHYL
205 Citations1985S. Carl Falco, Kathy S. Dumas
Several lines of evidence demonstrate that the SMR1 mutations are alleles of ILV2 which encodes acetolactate synthase (ALS), the target of SM, and that these mutations result in the production of ALS enzyme activity with increased resistance to SM.
Journal of BacteriologyYeast mutants auxotrophic for choline or ethanolamine
182 Citations1980K D Atkinson, Birgitte Nybo Jensen +4 more
Three mutants of the yeast Saccharomyces cerevisiae which require exogenous ethanolamine or choline were isolated and the lipid composition suggests that cho1 mutants do not synthesize phosphatidylserine under any growth conditions.
Methods in cell biologyChapter 12 Genetic Mapping in Yeast
173 Citations1975Robert Mortimer, D C Hawthorne
This chapter is devoted to genetic mapping in Saccharomyces cerevisiae ; however, the principles and techniques are generally applicable to investigations with other yeasts.
CellIsolation of a circular derivative of yeast chromosome III: implications for the mechanism of mating type interconversion
171 Citations1979Jeffrey N. Strathern, Carol S. Newlon +2 more
Genetic and physical characterization of rearrangements of chromosome III which result in changes of cell type in S. cerevisiae are described and it is suggested this deletion removes MATalpha and activates cryptic MATa information stored in HMalpha as proposed in the cassette model of mating type interconversion.
GeneticsIDENTIFICATION OF NEW GENES INVOLVED IN THE REGULATION OF YEAST ALCOHOL DEHYDROGENASE II
167 Citations1984Clyde L. Denis
A model of ADHII regulation is proposed in which both ADR1 and CCR4 are required forADHII expression, whereas CRE1 and CRE2 negatively control C CR4, whereas CCR1 is required for ADR 1 function.
Molecular and Cellular BiologyCloning and genetic mapping of SNF1, a gene required for expression of glucose-repressible genes in Saccharomyces cerevisiae.
164 Citations1984John L. Celenza, Marian Carlson
Journal of BacteriologyChromosomal superkiller mutants of Saccharomyces cerevisiae
158 Citations1978Akio Toh‐e, Patricia Guerry +1 more
It is found that recessive mutations in any of four chromosomal genes result in the superkiller phenotype, i.e., increased secretion of killer toxin activity by strains carrying the killer genome.
The EMBO JournalIdentification of two genes coding for the translation elongation factor EF‐1 alpha of S. cerevisiae.
151 Citations1984F. Schirmaier, Peter Philippsen
A search for TEF1 homologous sequences in several yeast species shows, in most cases, duplicated genes and a much higher sequence conservation than among genes encoding amino acid biosynthetic enzymes.
Molecular and General Genetics MGGIsolation and characterization of yeast mutants defective in intermediary carbon metabolism and in carbon catabolite derepression
143 Citations1977Michael Ciriacy
In ccr1 mutants, derepression of isocitrate lyase, fructose-1,6-diphosphatase, ADHII and possibly of the cytoplasmic MDH is prevented, whereas the mitochondrial TCA-cycle enzymes, succinate dehydrogenase and malate dehydrogenases, are not significantly affected.
Journal of Biological ChemistryIdentification of Saccharomyces cerevisiae mutants deficient in DNA topoisomerase I activity.
137 Citations1984C Thrash, K Voelkel +2 more
Mutants of the yeast, Saccharomyces cerevisiae, deficient in DNA topoisomerase I activity have been identified and the mutation has been mapped to MAK1, a gene required for maintenance of killer RNA.
Journal of Molecular BiologyA cis-dominant regulatory mutation linked to the argB-argC gene cluster in Saccharomyces cerevisiae
137 Citations1980Paul Jacobs, J C Jauniaux +1 more
New data are presented supporting the idea that two separate proteins are produced in vivo from the argB-argC gene cluster, and it is provided that the low levels of both enzyme activities in arginine-grown cells are due to repression rather than to inactivation of the enzymes.
Molecular and Cellular BiologyRegulation of glutamine-repressible gene products by the GLN3 function in Saccharomyces cerevisiae.
134 Citations1984Aaron P. Mitchell, Boris Magasanik
GeneticsGENES AFFECTING THE EXPRESSION OF CYTOCHROME <i>c</i> IN YEAST: GENETIC MAPPING AND GENETIC INTERACTIONS
134 Citations1980Rodney Rothstein, Fred Sherman
It is suggested that cyc2 and cyc3 may be involved with the regulation or maturation of the iso-cytochromes c, which is associated with flocculent cells and other abnormal phenotypes.
GeneticsTWO CHROMOSOMAL GENES REQUIRED FOR KILLING EXPRESSION IN KILLER STRAINS OF <i>SACCHAROMYCES CEREVISIAE</i>
132 Citations1976Reed B. Wickner, Michael J. Leibowitz
28 independent recessive chromosomal mutants of a killer strain that have lost the ability to secrete an active toxin but remain resistant to the effects of the toxin and continue to carry the complete cytoplasmic killer genome are isolated.
Journal of BacteriologyMutants of <i>Saccharomyces cerevisiae</i> That Incorporate Deoxythymidine-5′-Monophosphate Into Deoxyribonucleic Acid In Vivo
131 Citations1974Reed B. Wickner
Spontaneous mutants of Saccharomyces cerevisiae able to incorporate deoxythymidine-5'-monophosphate (dTMP) into deoxyribonucleic acid (DNA) have been selected based on their ability to grow in the presence of aminopterin and sulfanilamide if dTMP is present, and incorporation was sufficient to account for a substantial proportion of the thymine residues in the cellular DNA.
Journal of BacteriologyUnsaturated Fatty Acid Mutants of <i>Saccharomyces cerevisiae</i>
130 Citations1966Michael A. Resnick, Robert Mortimer
Two types of lipid nutritional mutants have been induced in this organism and one of these classes of mutants requires an unsaturated fatty acid and is associated with a locus on chromosome VII and the other class of mutants needs either an uns saturated fatty acid or ergosterol for growth.
GeneticsA NEW MAPPING METHOD EMPLOYING A MEIOTIC REC- MUTANT OF YEAST
130 Citations1982Sue Klapholz, Rochelle Easton Esposito
The utility of this mapping procedure is demonstrated by confirming the chromosomal location of seven known markers, as well as by the assignment of a previously unmapped mutation, spo12-1, to chromosome VIII.
GeneticsMEIOTIC GENE CONVERSION IN YEAST TETRADS AND THE THEORY OF RECOMBINATION
130 Citations1967Seymour Fogel, Donald D Hurst
GeneticsGENETIC MAPPING OF NONSENSE SUPPRESSORS IN YEAST
128 Citations1968D C Hawthorne, Robert Mortimer
Journal of Molecular BiologyDetermination of the nuclear DNA content of Saccharomyces cerevisiae and implications for the organization of DNA in yeast chromosomes
125 Citations1977Gail D. Lauer, Thomas M. Roberts +1 more
V viscoelastic measurements on yeast DNA are now consistent with the idea that each chromosome contains one piece of DNA.
CellGenetic properties of chromosomally integrated 2μ plasmid DNA in yeast
118 Citations1982S. Carl Falco, Yuyang Li +2 more
It is proposed that the presence of integrated 2 mu DNA allows efficient integration of additional copies of the intact 2 mu plasmid by the action of the plasmids-coded special recombination system.
Molecular and Cellular BiologyAsparagine-linked glycosylation in Saccharomyces cerevisiae: genetic analysis of an early step.
115 Citations1984Georjana Barnes, William J. Hansen +2 more
Molecular and Cellular BiologyIdentification of the genetic locus for the structural gene and a new regulatory gene for the synthesis of repressible alkaline phosphatase in Saccharomyces cerevisiae.
113 Citations1982Yoshinobu Kaneko, Akio Toh‐e +1 more
GeneticsGENETICS OF YEAST HEXOKINASE
110 Citations1977Zita Lobo, P.K. Maitra
Two independent isolates of Saccharomyces cerevisiae lacking hexokinase activity are described, and genetic analysis suggests that these two unlinked genes hxk1 and hxK2 determine, independently of each other, the synthesis ofhexokinase isozymes P1 and P2, respectively.
Molecular and General Genetics MGGMethionine biosynthesis in Saccharomyces cerevisiae
109 Citations1975Monique Masselot, Huguette de Robichon-Szulmajster
To analyse how many structural genes are implicated in the specifi steps of the biosynthesis of methionine in Sacch.
Journal of Molecular BiologyChromosomal genes essential for replication of a double-stranded RNA plasmid of Saccharomyces cerevisiae: The killer character of yeast
103 Citations1976Reed B. Wickner, Michael J. Leibowitz
Strains of Saccharomyces cerevisiae carrying a small double-stranded RNA species (the killer plasmid) secrete a toxin which is lethal only to strains not carrying this plasmids, and isolated mutants in eight chromosomal genes essential for replication or maintenance of the killer Plasmid.
Journal of BacteriologyMutants of Yeast Sensitive to Ultraviolet Light
103 Citations1967Richard Snow
Six uvr mutants of Saccharomyces cerevisiae with hypersensitivity to ultraviolet (UV) light were isolated after mutagen treatment with ethylmethanesulfonate and implementation tests and tetrad analysis indicate that each strain represents mutation in a different gene.
Proceedings of the National Academy of SciencesMitotic chromosome loss in a radiation-sensitive strain of the yeast Saccharomyces cerevisiae.
103 Citations1981Robert Mortimer, R. Contopoulou +1 more
It is shown that diploid cells homozygous for rad52 lose chromosomes at high frequencies and that these frequencies of loss can be increased dramatically by exposure of these cells to x-rays.
Molecular and Cellular BiologyRegulation of CDC9, the Saccharomyces cerevisiae gene that encodes DNA ligase.
102 Citations1985Thomas Peterson, Louise Prakash +3 more
GeneticsFRAMESHIFTS AND FRAMESHIFT SUPPRESSORS IN <i>SACCHAROMYCES CEREVISIAE</i>
101 Citations1977Michael R. Culbertson, Lawrence Charnas +2 more
Using ICR-170 as a mutagen, a set of mutations in yeast which exhibit behavior similar to that shown for bacterial frameshift mutations are induced, showing that these mutations are polar.
Journal of Molecular BiologyIdentification of the yeast DNA sequences that correspond to specific tyrosine-inserting nonsense suppressor loci
101 Citations1979Maynard V. Olson, Kate Loughney +1 more
GeneticsFRAMESHIFT SUPPRESSION IN <i>SACCHAROMYCES CEREVISIAE</i>. II. GENETIC PROPERTIES OF GROUP II SUPPRESSORS
95 Citations1980Michael R. Culbertson, Karen M Underbrink +1 more
Suppressors of ICR-induced mutations that exhibit behavior similar to bacterial frameshift suppressors have been identified in the yeast Saccharomyces cerevisiae andMethods that permit positive selection have been devised in order to examine large numbers of variants.
Journal of BacteriologyIsolation and characterization of Saccharomyces cerevisiae mutants deficient in S-adenosylmethionine decarboxylase, spermidine, and spermine
93 Citations1978M.S. Cohn, C W Tabor +1 more
Evidence was obtained that arg1 was not located on any of the 17 known chromosomes, and therefore it is postulate that arg 1 and spe2 are located on a new 18th chromosome.
CellAn extensive deletion causing overproduction of yeast iso-2-cytochrome c
91 Citations1981Gary McKnight, Thomas S. Cardillo +1 more
A cis-dominant regulatory mutation that causes a 20-fold overproduction of yeast iso-2-cytochrome c and a gene cluster that displays striking similarity to the gene cluster CYC1-OSM1-RAD7, which controls iso-1-cy tochrome c, osmotic sensitivity and UV sensitivity are suggested.
GeneticsMITOTIC RECOMBINATION AND HETEROALLELIC REPAIR IN SACCHAROMYCES CEREVISIAE
89 Citations1964Donald D Hurst, Seymour Fogel
The idea that prototroph formation might result from both equal and unequal crossing over between homologues, and from intrachromosomal exchange mechanisms that do not involve reciprocal double mutant expected of classical recombination is suggested.
Journal of BacteriologyCryptopleurine Resistance: Genetic Locus for a 40 <i>S</i> Ribosomal Component in <i>Saccharomyces cerevisiae</i>
89 Citations1974P G Grant, Lucas Sánchez +1 more
Mutants resistant to the phenanthrene alkaloids tylophorine, tylocrebrine, and cryptopleurine have been isolated from the yeast Saccharomyces cerevisiae and the mode of action has been examined, and they appear to inhibit the translocation phase of protein synthesis.
GeneticsMAXIMUM LIKELIHOOD ESTIMATION OF LINKAGE AND INTERFERENCE FROM TETRAD DATA
87 Citations1979Richard Snow
The difference in interference characteristics of S. cerevisiae and Schizosaccharomyces pombe seems to be a reflection of some fundamental difference in the recombination process of the two species.
GeneticsA RAPID CHROMOSOME-MAPPING METHOD FOR CLONED FRAGMENTS OF YEAST DNA
87 Citations1983S. Carl Falco, David Botstein
After verification of the accuracy of the method using several genes whose position was known in advance, the method was used to locate the yeast actin gene, which lies on the left arm of chromosome VI, about 50 cM distal to CDC4.
Journal of BacteriologyIsolation and characterization of aminopeptidase mutants of Saccharomyces cerevisiae
84 Citations1983Robert Trumbly, Grant Bradley
Mutants of Saccharomyces cerevisiae were isolated which have decreased ability to hydrolyze leucine beta-naphthylamide, a chromogenic substrate for amino-peptidases, and mutations were shown by starch gel electrophoresis to affect one of four different aminopeptid enzymes.
GeneticsCHARACTERIZATION OF A MUTATION IN YEAST CAUSING NONRANDOM CHROMOSOME LOSS DURING MITOSIS
83 Citations1978Paloma Liras, John H. McCusker +2 more
The chl mutation does not cause an increase in spontaneous mutations, nor are mutant strains sensitive to UV or irradiation, but a decrease in chromosome III linkage relationships is also found.
Journal of BacteriologyUninducible Mutants in the <i>gal i</i> Locus of <i>Saccharomyces cerevisiae</i>
82 Citations1972H. C. Douglas, D C Hawthorne
The susceptibility to suppression by an ochre suppressor of an i allele in an i(s)i(-) double mutant suggests that the i locus in S. cerevisiae codes for a protein.
Journal of Molecular BiologySerine substitutions caused by an ochre suppressor in yeast
82 Citations1975Susan W. Liebman, John Stewart +1 more
SUQ5 differs from the eight other characterized suppressors of UAA in yeast, which were previously shown to insert residues of tyrosine at ochre sites and which are only effective in strains haying the non-Mendelian factor ψ−, since they generally cause inviability in the ψ+ state.
GeneticsTHE ROLE OF <i>S. CEREVISIAE</i> CELL DIVISION CYCLE GENES IN NUCLEAR FUSION
81 Citations1982Susan K. Dutcher, Leland H. Hartwell
Forty temperature-sensitive cell division cycle (cdc) mutants of Saccharomyces cerevisiae were examined for their ability to complete nuclear fusion during conjugation in crosses to a CDC parent strain at the restrictive temperature and it was found that both defects resulted from a single lesion for each of these cdc mutants.
Molecular and General Genetics MGGGenetic and biochemical characterization of mutants of Saccharomyces cerevisiae blocked in six different steps of heme biosynthesis
80 Citations1981Danièle Urban‐Grimal, Rosine Labbe‐Bois
Heme-deficient mutants of Saccharomyces cerevisiae have been isolated from two isogenic strains with the use of an enrichment method based on photodynamic properties of Zn-protoporphyrin to discuss in terms of possible modes of regulation of heme synthesis pathway in yeast.
Journal of BacteriologyModification of Ribosomes in Cryptopleurine-Resistant Mutants of Yeast
79 Citations1973Lawrence Skogerson, Calvin S. McLaughlin +1 more
Analysis of the distribution of ribosomes between monosomes and polysomes in sensitive cells exposed to cryptopleurine suggests that some step is inhibited during the elongation phase of protein synthesis.
GeneticsPLEIOTROPIC MUTATIONS AT THE <i>TUP1</i> LOCUS THAT AFFECT THE EXPRESSION OF MATING-TYPE-DEPENDENT FUNCTIONS IN <i>SACCHAROMYCES CEREVISIAE</i>
76 Citations1980Jeffrey F. Lemontt, Donna R Fugit +1 more
It is suggested that the product of this gene plays some common central role in the complex regulation of the expression of both MAT-dependent and MAT-independent functions in heterothallic Saccharomyces cerevisiae.
Cold Spring Harbor Symposia on Quantitative BiologyCharacterization of the Yeast Mobile Element Tyl
76 Citations1981Hermann Eibel, J. Gafner +2 more
GeneticsTWENTY-SIX CHROMOSOMAL GENES NEEDED TO MAINTAIN THE KILLER DOUBLE-STRANDED RNA PLASMID OF <i>SACCHAROMYCES CEREVISIAE</i>
75 Citations1978Reed B. Wickner
A new series of mutants in chromsomal genes needed for killer plasmid maintenance (mak genes) are described, which comprise 12 complementation groups and consist of at least 26 mak genes.
Journal of Biological ChemistryCloning and expression in Escherichia coli of a yeast mannosyltransferase from the asparagine-linked glycosylation pathway.
74 Citations1984J Couto, Tim C. Huffaker +1 more
The cloned gene ALG1 has been cloned by complementation of the temperature-sensitive mutation alg1-1 with a total genomic DNA library and is proved to be the structural gene for the first mannosyltransferase in lipid-linked oligosaccharide assembly.
GeneticsGENE DUPLICATION IN <i>SACCHAROMYCES CEREVISIAE</i>
74 Citations1978Paul E. Hansche, V. Beres +1 more
Five independent duplications of the aphtase structural gene were recovered from chemostat populations of S. cerevisiae that were subject to selection for in vivo hyper-aphtase activity, and one of the five duplicated regions of the right arm of chromosome II was found to be transmitted mitotically and meiotically with very high fidelity.
Molecular and General Genetics MGGStudies of the genetic mechanism of radiation-induced mitotic segregation in yeast
71 Citations1969Sayaka Nakai, Robert Mortimer
Sectoring was induced with x-rays or ultraviolet in a diploid yeast strain heterozygous for seven genes located on one chromosome arm and could be explained single mitotic crossovers in one of the intergenic regions.
Molecular and Cellular BiologyIsolation and characterization of dominant mutations resistant to carbon catabolite repression of galactokinase synthesis in Saccharomyces cerevisiae.
70 Citations1981Kengo Matsumoto, Akio Toh‐e +1 more
GeneticsA NEW GENE AFFECTING THE EFFICIENCY OF MATING-TYPE INTERCONVERSIONS IN HOMOTHALLIC STRAINS OF <i>SACCHAROMYCES CEREVISIAE</i>
70 Citations1977James E. Haber, Barbara Garvik
A mutant homothallic strain that gives rise to colonies that show bisexual mating behavior is isolated, and the switch mutation does not affect the efficiency of self-mating, but rather directly affects the frequency of interconversion of mating types.
Journal of BacteriologyGenetic Order of the Galactose Structural Genes in <i>Saccharomyces cerevisiae</i>
67 Citations1971John Bassel, Robert Mortimer
The galactose structural genes of Saccharomyces cerevisiae were ordered by determining the genotypes of mitotic and meiotic recombinants from crosses heterozygous for the three genes, and the most probable order is centromere-gal7-gal10-gal1.
Canadian Journal of MicrobiologyThe identification, characterization, and mapping of a gene for flocculation in <i>Saccharomyces</i> sp.
66 Citations1977Graham G. Stewart, I. Russell
Mapping studies of this flocculation gene have revealed that it is linked to ade 1 and therefore located on Chromosome I, and this is a different gene to the threeflocculation genes studied by other laboratories because they have found such genes to be unlinked toade 1.
Journal of BacteriologySterol methylation in Saccharomyces cerevisiae
65 Citations1984Mark T. McCammon, Michael Hartmann +2 more
Various nystatin-resistant mutants defective in S-adenosylmethionine: delta 24-sterol-C-methyltransferase were shown to possess alleles of the same gene, erg6, and the genetic map location of erg6 was shown to be close to trp1 on chromosome 4.1.
Proceedings of the National Academy of SciencesRibosomal protein L3 is involved in replication or maintenance of the killer double-stranded RNA genome of Saccharomyces cerevisiae.
65 Citations1982Reed B. Wickner, S P Ridley +2 more
It is concluded that ribosomal protein L3 is involved in the replication and maintenance of M ds RNA, and therefore MAK8 and TCM1 are the same gene.
GeneticsRECOMBINATION BETWEEN GENES LOCATED ON NONHOMOLOGOUS CHROMOSOMES IN <i>SACCHAROMYCES CEREVISIAE</i>
65 Citations1982Margaret Dubay Mikus, Thomas D. Petes
It is shown that Leu+ or Ura+ revertants can arise by a variety of different genetic interactions, and reciprocal recombination between repeated genes, resulting in reciprocally translocated chromosomes is detected.
Journal of BacteriologyMeiotic mapping of yeast ribosomal deoxyribonucleic acid on chromosome XII
65 Citations1979Thomas D. Petes
Using meiotic mapping techniques to locate the position of the repeating ribosomal DNA (rDNA) genes of the yeast Saccharomyces cerevisiae, it is found that the rDNA genes are located on the right arm of chromosome XII, approximately 45 map units centromere distal to the gene gal2.
Cold Spring Harbor Monograph ArchiveGenetic Mapping in Saccharomyces cerevisiae
65 Citations1981Robert Mortimer, David Schild
Both meiotic and mitotic approaches have been developed to map yeast genes and the various techniques used to locate genes on the genetic map of the yeast S. cerevisiae will be reviewed.
GeneticsMAPPING OF TRICHODERMIN RESISTANCE IN <i>SACCHAROMYCES CEREVISIAE</i>: A GENETIC LOCUS FOR A COMPONENT OF THE 60S RIBSOMAL SUBUNIT
62 Citations1976P G Grant, Daniel Schindler +1 more
The resistance locus, tcm1 was found to be closely linked (1 centi-morgan) to the locus pet 17 on the right arm of chromosome XV, which conferred resistance to other 12,13-epoxytrichothecenes and to the structurally unrelated antibiotic anisomycin.
Molecular and General Genetics MGGYeast cell-cycle mutantcdc21 is a temperature-sensitive thymidylate auxotroph
61 Citations1976John C. Game
Genetic tests with the yeast cell-cycle mutantcdc21 isolated by Hartwell indicate that the CDC21 gene in yeast is the same as the TMP1 gene, whose mutant alleles confer an auxotrophic requirement for thymidine-5′-monophosphate (dTMP).
Carlsberg Research CommunicationsA mutant of Saccharomyces cerevisiae temperature sensitive for flocculation. Influence of oxygen and respiratory deficiency on flocculence
60 Citations1978Steen Holmberg, Morten C. Kielland‐Brandt
A method for measuring flocculence using the spectrophotometer is described and a number of petites induced in the mutant strain with ethidium bromide had altered flocculation phenotypes.
Journal of BacteriologyMak mutants of yeast: mapping and characterization
59 Citations1979Reed B. Wickner, Michael J. Leibowitz
This work has mapped mutations in eight of the chromosomal genes needed for maintaining M (mak genes), and shows that mutants defective in these and other mak genes lose M ds RNA, but not L dsRNA.
Molecular and Cellular BiologyProline Utilization in <i>Saccharomyces cerevisiae</i> : Analysis of the Cloned <i>PUT2</i> Gene
59 Citations1983M C Brandriss
The PUT2 gene was isolated on a 6.5-kilobase insert of a recombinant DNA plasmid by functional complementation of a put2 (delta 1-pyrroline-5-carboxylate dehydrogenase-deficient) mutation in Saccharomyces cerevisiae by a gene disruption technique.
GeneticsTEMPERATURE-SENSITIVE LETHAL MUTATIONS ON YEAST CHROMOSOME <i>I</i> APPEAR TO DEFINE ONLY A SMALL NUMBER OF GENES
59 Citations1984David B. Kaback, Paul W. Oeller +5 more
Molecular and Cellular Biologyrme1 Mutation of Saccharomyces cerevisiae: map position and bypass of mating type locus control of sporulation.
58 Citations1981Jasper Rine, G. F. Sprague +1 more
GeneticsMAPPING AND GENE CONVERSION STUDIES WITH THE STRUCTURAL GENE FOR ISO-1-CYTOCHROME <i>C</i> IN YEAST
56 Citations1975Christopher W. Lawrence, Fred Sherman +2 more
The frequencies of recombination and the occurrence of coconversions suggest that these four genes are contiguous or at least nearly so, and the orientation of the Cyc1 gene could not be clearly deduced from the behavior of the distal marker SUP4 in wild-type recombinants that arose from diploids heteroallelic for cyc1 mutations.
CellAnalysis of the junction between ribosomal RNA genes and single-copy chromosomal sequences in the yeast Saccharomyces cerevisiae
56 Citations1982Timothy J. Zamb, Thomas D. Petes
The yeast Saccharomyces cerevisiae has a single tandem array of 100 ribosomal RNA (rRNA) genes and a junction between the centromere-distal end of this array and the adjacent single-copy chromosomal sequences is cloned and characterized.
GeneticsRADIATION-INDUCED GENETIC SEGREGATIONS IN VEGETATIVE CELLS OF DIPLOID YEAST
55 Citations1955A. P. James, Brenda Lee-Whiting
The present investigation was undertaken to obtain information on the mechanism by which the segregants are produced and to determine the carrier and removal status of these carriers.
GeneticsPLEIOTROPIC PROPERTIES OF A YEAST MUTANT INSENSITIVE TO CATABOLITE REPRESSION
54 Citations1980Helene Cherrick Stark, Donna R Fugit +1 more
Results suggest that the flk1 mutation is not a specific lesion affecting catabolite repression, and is likely to be allelic to the pleiotropic mutants tup1, cyc9, and umr7.
GeneticsDELETIONS OF THE ISO-1-CYTOCHROME <i>c</i> AND ADJACENT GENES OF YEAST: DISCOVERY OF THE <i>OSM1</i> GENE CONTROLLING OSMOTIC SENSITIVITY
52 Citations1978Arjun Singh, Fred Sherman
Some of the deletions in the yeast Saccharomyces cerevisiae that encompass the CYC 1 gene, which determines iso-1-cytochrome c, extend into the OSM1 gene, causing inhibition of growth on hypertonic media, and into the RAD7 genes, causing sensitivity to UV light.
Journal of BacteriologyIsolation and characterization of Saccharomyces cerevisiae glycolytic pathway mutants
52 Citations1977Keng-Bon Lam, Julius Marmur
Yeast strains carrying recessive mutations representing four different loci that cause defects in pyruvate kinase, pyruVate decarboxylase, 3-phosphoglycerate Kinase, and 3- phosphoglycerates mutase were isolated and partially characterized.
GeneticsMAPPING OF THE HOMOTHALLIC GENES, <i>HMα</i> AND <i>HM</i> <b>a</b>, IN SACCHAROMYCES YEASTS
51 Citations1976Satoshi Harashima, Yasuji Oshima
Two of the three homothallic genes, HM alpha and HMa, showed direct linkage to the mating-type locus at approximately 73 and 98 strans (57 and 65 centimorgans [cM], respectively, respectively) whereas, the other, HO, showed no linkage to 25 standard markers distributed over 17 chromosomes including the mateable locus.
GeneticsSTRUCTURAL ANALYSIS OF THE <i>dur</i> LOCI IN <i>S. CEREVISIAE</i>: TWO DOMAINS OF A SINGLE MULTIFUNCTIONAL GENE
51 Citations1980Terrance Cooper, Christine Lam +1 more
Data suggest that the two dur loci might in reality be domains of a single gene that codes for a multifunctional polypeptide in Saccharomyces cerevisiae.
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