Persistent Site-Specific Remodeling of a Nucleosome Array by Transient Action of the SWI/SNF Complex
SciencePublished 26 July 1996
Tom Owen‐Hughes, Rhea T. Utley, Jacques Côté, Craig L. Peterson, Jerry L. Workman
Citations224
SJR quartileQ1
SJR score10.42
SNIP6.62
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TL;DR
Analysis of individual nucleosomes revealed that the transient action of the SWI/SNF complex catalyzed eviction of histones from the Gal4-bound nucleosome that was bound by derivatives of the transcription factor Gal4p.
Abstract
The SWI/SNF complex participates in the restructuring of chromatin for transcription. The function of the yeast SWI/SNF complex in the remodeling of a nucleosome array has now been analyzed in vitro. Binding of the purified SWI/SNF complex to a nucleosome array disrupted multiple nucleosomes in an adenosine triphosphate-dependent reaction. However, removal of SWI/SNF left a deoxyribonuclease I-hypersensitive site specifically at a nucleosome that was bound by derivatives of the transcription factor Gal4p. Analysis of individual nucleosomes revealed that the SWI/SNF complex catalyzed eviction of histones from the Gal4-bound nucleosomes. Thus, the transient action of the SWI/SNF complex facilitated irreversible disruption of transcription factor-bound nucleosomes.
Keywords
MedicineBiochemistry, Genetics and Molecular Biology
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