Molecular mechanism of genetic recombination in bacterial transformation
Molecular Genetics and GenomicsPublished 1 January 1964
S. E. Bresler, Р. А. Кренева, V. V. Kushev, M. I. Mosevitski�
Citations16
SJR quartileQ2
SJR score0.55
SNIP0.69
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Abstract
B. subtilis cells auxotrophic for two linked markers (ind-his, ind-tyr, his-tyr) have been transformed by means of DNA preparations obtained by hybridization of wild type DNA with the DNA of a strain auxotrophic for one of the linked markers. It was established that hybridization does not increase the transforming activity of DNA for the heterozygous marker. A genetic analysis of the progeny of cells transformed by hybrid or wild type DNA was performed. On the basis of the data obtained a model of genetic recombination in transformation is proved. According to this model both strands of the donor DNA interact independently with the chromosome, and either strand can be incorporated into the cell genome with equal probability. According to the estimate made on the basis of this hypothesis, the probability of integration of a single DNA strand carrying a particular genetic marker is 8%.
Keywords
Biochemistry, Genetics and Molecular BiologyEnvironmental Science
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