The <i>tol</i> gene products and the import of macronmolecules into <i>Escherichia coli</i>
Molecular MicrobiologyPublished 1 May 1991Open access
Robert E. Webster
Citations253
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SJR score1.25
SNIP0.83
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TL;DR
A preliminary model of the location and possible interactions of the Tol proteins is presented and information is also available regarding the domains of the colicins or phage proteins which interact with the Tolprotein.
Abstract
Genetic studies have identified a number of genes whose products appear to be required for the transport of the group A colicins and the single-stranded DNA of certain filamentous bacteriophages into Escherichia coli. Mutations in these genes allow normal binding of the colicins to their outer-membrane receptors and of the bacteriophage of the tip of specific conjugative pili, but do not allow translocation of the macromolecules to their target. These mutations have been designed 'tolerant' (tol) mutations and the protein products specified by these genes appear to comprise part of a transport system known as the Tol import system. Some of these genes have been isolated, sequenced and their protein products localized to the membranes or periplasm of E. coli. Information is also available regarding the domains of the colicins or phage proteins which interact with the Tol proteins. A preliminary model of the location and possible interactions of the Tol proteins is presented.
Keywords
Biochemistry, Genetics and Molecular BiologyEnvironmental Science
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