The suppression of long-term potentiation in rat hippocampus by inhibitors of nitric oxide synthase is temperature and age dependent
NeuronPublished 1 November 1993
John Williams, Y.‐G. Li, A. Nayak, M.L. Er̀rington, Kerry Murphy, T.V.P. Bliss
Citations216
SJR quartileQ1
SJR score6.75
SNIP2.95
Generate an AI Snapshot to get a quick, structured summary of this paper.
Study Snapshot
ObjectiveStudy objective
MethodsResearch methodology
PopulationPopulation studied
Sample sizeSample sizes
OutcomesStudy outcomes here
ResultsStudy results comes here
LimitationsResearch study limitations comes here
A concise AI-generated summary of the paper will appear here once you click Generate AI Snapshot.
TL;DR
Neither L-NA nor hemoglobin had any significant effect on LTP in slices from adult rats at room temperature, or in young rats at 29 degrees C-30 degrees C, suggesting that nitric oxide is unlikely to play a role in the induction of LTP under physiological conditions.
Abstract
At room temperature (23 degrees C-25 degrees C), the induction of long-term potentiation (LTP) in area CA1 of slices from young male Sprague-Dawley rats was depressed by preincubation with the nitric oxide synthase inhibitors NG-nitro-L-arginine (L-NA, 100 microM) and NG-nitro-L-arginine methyl ester (L-NAME, 100 microM). The D isomers were ineffective under the same conditions. Hemoglobin (20 microM) reduced but did not completely block LTP. Neither L-NA (at concentrations up to 1 mM) nor hemoglobin (20 microM) had any significant effect on LTP in slices from adult rats at room temperature, or in young rats at 29 degrees C-30 degrees C. These results suggest that nitric oxide is unlikely to play a role in the induction of LTP under physiological conditions.
Keywords
MedicineNeuroscience
NatureA synaptic model of memory: long-term potentiation in the hippocampus
11,542 Citations1993T.V.P. Bliss, Graham L. Collingridge
The best understood form of long-term potentiation is induced by the activation of the N-methyl-d-aspartate receptor complex, which allows electrical events at the postsynaptic membrane to be transduced into chemical signals which, in turn, are thought to activate both pre- and post Synaptic mechanisms to generate a persistent increase in synaptic strength.
ScienceA Requirement for the Intercellular Messenger Nitric Oxide in Long-Term Potentiation
1,150 Citations1991Erin M. Schuman, Daniel V. Madison
Findings suggest that nitric oxide liberated from postsynaptic neurons may travel back to presynaptic terminals to cause LTP expression.
Proceedings of the National Academy of SciencesTests of the roles of two diffusible substances in long-term potentiation: evidence for nitric oxide as a possible early retrograde messenger.
1,042 Citations1991Thomas J. O’Dell, Robert D. Hawkins +2 more
Two major membrane-permeant candidate retrograde messengers are investigated, arachidonic acid and nitric oxide, and no enhances spontaneous presynaptic release of transmitter from hippocampal neurons in dissociated cell culture, suggesting that NO might be a retrograde messenger in LTP.
Brain ResearchPatterned stimulation at the theta frequency is optimal for the induction of hippocampal long-term potentiation
1,039 Citations1986John Larson, Darryl Wong +1 more
The results indicate that patterns of stimulation resembling spike discharge patterns of hippocampal neurons in animals in exploratory situations are effective in inducing LTP and suggest temporal constraints on the mechanisms involved in triggering synaptic plasticity.
NeuronEffect of nitric oxide production on the redox modulatory site of the NMDA receptor-channel complex
749 Citations1992Sizheng Z. Lei, Zhuo-Hua Pan +5 more
The results demonstrate a new pathway for NO regulation of physiological function that is not via cGMP, but instead involves reactions with membrane-bound thiol groups on the NMDA receptor-channel complex.
European Journal of PharmacologyPossible involvement of nitric oxide in long-term potentiation
634 Citations1991Georg Andrees Böhme, Christelle L. M. Bon +3 more
It is reported here that the NO-synthase inhibitor, L-N omega-nitro-arginine, blocks LTP and that sodium nitroprusside, which releases NO, produces a long-lasting enhancement in synaptic efficacy which is not additive with tetanus-induced LTP.
ScienceNitric Oxide And Carbon Monoxide Produce Activity-Dependent Long-Term Synaptic Enhancement in Hippocampus
556 Citations1993Min Zhuo, Scott A. Small +2 more
Results are consistent with the hypothesis that NO and CO, either alone or in combination, serve as retrograde messages that produce activity-dependent presynaptic enhancement during LTP.
NeuronThe role of nitric oxide in hippocampal long-term potentiation
470 Citations1992Jane E. Haley, George L. Wilcox +1 more
It is reported that only a relatively short-term potentiation can be induced in the presence of nitro-L-arginine methyl ester (L-NAME), an NO synthase inhibitor, and a role for NO is suggested in the maintenance of activity-dependent synaptic enhancements, possibly via the generation of cGMP.
NeuronNitric oxide-induced blockade of NMDA receptors
452 Citations1992Olivier J. Manzoni, Laurent Prézeau +4 more
Results suggest a feedback inhibition of NMDA receptors by NO under physiological condition and Pretreatment with hemoglobil potentiated NMDA-induced effects, demonstrating that endogenous NO modulatesNMDA receptors.
ScienceThe Influence of Prior Synaptic Activity on the Induction of Long-Term Potentiation
416 Citations1992Yan-You Huang, A. Colino +2 more
The results suggest that the threshold for LTP induction may be continually adjusted according to the recent history of NMDA receptor activation and provide a physiological mechanism by which LTP can be transiently inhibited.
NeuronMechanisms generating the time course of dual component excitatory synaptic currents recorded in hippocampal slices
397 Citations1990Shaul Hestrin, Pankaj Sah +1 more
The present results support a model in which the rise time and decay of the NMDA component are determined primarily by slow channel kinetics and the Decay of the non-NMDA component is due either to channel Kinetics or to desensitization.
NatureFacilitated induction of hippocampal long-lasting potentiation during blockade of inhibition
354 Citations1983Holger Wigström, B. Gustafsson
Study of long-lasting potentiation in the hippocampal slice preparation (CA1 region) in conditions where postsynaptic inhibition was reduced by application of γ-aminobutyric acid (GABA) receptor blockers found that in addition to their direct effect on inhibition, GABA blockers dramatically facilitated the induction ofLong- lasting potentiation.
Journal of NeuroscienceTemperature dependence of intrinsic membrane properties and synaptic potentials in hippocampal CA1 neurons in vitro
297 Citations1985SM Thompson, LM Masukawa +1 more
Reductions in temperature of as little as 5 to 10 degrees C from normal can significantly alter the intrinsic and synaptic physiology of hippocampal neurons and should, therefore, be considered an important variable in in vitro brain slice experiments.
Neuroscience LettersNMDA receptor activation in rat hippocampus induces cyclic GMP formation through the l-arginine-nitric oxide pathway
296 Citations1991Stephen J. East, John Garthwaite
The results indicate that NMDA receptor activation in the hippocampus induces the generation of nitric oxide from arginine and that this novel intercellular messenger mediates the increases in cyclic GMP levels.
The Journal of PhysiologyCorrelation between long‐term potentiation and release of endogenous amino acids from dentate gyrus of anaesthetized rats.
287 Citations1986T.V.P. Bliss, R. M. Douglas +2 more
The origin of the relative increases in the concentration of glutamate and aspartate which are found in the perfusate following the induction of l.t.p. may be a sustained increase in activity‐dependent release of these amino acids from perforant path terminals as well as a decrease in the release of GABA.
ScienceInhibition of Long-Term Potentiation by NMDA-Mediated Nitric Oxide Release
233 Citations1992Yukitoshi Izumi, David B. Clifford +1 more
Results indicate that the timing of NO release relative to high-frequency activation of CA1 synapses may be an important determinant of LTP generation and suggest that NO may play a positive or negative modulatory role in LTP depending on prior events at the tetanized synapse and the ambient concentration of excitatory amino acids.
Neuroscience LettersLow-frequency activation of the NMDA receptor system can prevent the induction of LTP
157 Citations1989E.J. Coan, Andy J. Irving +1 more
It is suggested that certain types of activation of the NMDA receptor system can disable the LTP induction mechanism in rats bathed in Mg2(+)-free medium tetanic stimulation.
The Journal of PhysiologyInhibitory post‐synaptic currents in rat hippocampal CA1 neurones.
152 Citations1984G.L. Collingridge, Peter W. Gage +1 more
Spontaneous synaptic currents were recorded in voltage‐clamped CA1 neurones in rat hippocampal slices at room temperature and it was concluded that they were inhibitory post‐synaptic currents generated by the opening of chloride‐selective channels activated by gamma‐aminobutyric acid (GABA).
European Journal of NeuroscienceA Role for Nitric Oxide in Long‐term Potentiation
147 Citations1992Christelle L. M. Bon, Georg Andrees Böhme +3 more
Evidence is provided that endogenous nitric oxide production is necessary for synaptic plasticity in the CA1 hippocampus of the rat and that release locally from hydroxylamine produced a stable potentiation of synaptic transmission that was not additive with LTP induced by high‐frequency stimulation.
NeuroreportNitric oxide synthase-independent long-term potentiation in area CA1 of hippocampus
120 Citations1993Dane M. Chetkovich, Eric Klann +1 more
The findings demonstrate that while NO is elevated with LTP-inducing stimuli, the activity of NO synthase is not necessary for induction of LTP by all stimulus paradigms, and support the hypothesis that NO can function as a transcellular messenger in the hippocampus.
Brain ResearchEffects of high-frequency synaptic stimulation on glumate receptor binding studied with a modified in vitro hippocampal slice preparation
119 Citations1982Gary Lynch, Shelley Halpain +1 more
It is shown that the minislice preparation should be of general utility in relating synaptic physiology to synaptic chemistry and suggested that glutamate binding sites are regulated by patterns of afferent activity.
Neuroscience LettersNitric oxide synthase inhibitors block long-term potentiation induced by weak but not strong tetanic stimulation at physiological brain temperatures in rat hippocampal slices
102 Citations1993Jane E. Haley, Peter Malen +1 more
Results suggest that nitric oxide produced as a result of tetanic stimulation plays a role in adjusting the threshold of LTP induction, but is not necessary for establishing synaptic enhancement under conditions of strong synaptic activation.
Journal of NeurophysiologyEvidence for nitric oxide synthase inhibitor-sensitive and insensitive hippocampal synaptic potentiation
95 Citations1992Valentin K. Gribkoff, J T Lum-Ragan
Journal of NeurophysiologyNitric oxide inhibitors facilitate the induction of hippocampal long-term potentiation by modulating NMDA responses
52 Citations1993K. Katō, C. F. Zorumski
It is suggested that tonically released NO modulates the threshold for LTP in the CA1 hippocampal region and is consistent with prior studies indicating that untimely activation of NMDA receptors and release of NO inhibit LTP.
Seminars in NeuroscienceNitric oxide as an intercellular signal in long-term potentiation
20 Citations1993Erin M. Schuman, Daniel V. Madison
The diffusible nature of NO suggests the possibility that it may influence multiple synapses near the site of its generation, and a potential molecular effector of NO during LTP may be a cytosolic ADP-ribosyltransferase.
Brain ResearchAge-related effects of NMDA-stimulated concomitant release of nitric oxide and glutamate in cortical slices prepared from DBA/2 mice
14 Citations1993Helen L. Rowley, Y. Ellis +1 more
Results support a neuromodulator role for NO in the release of transmitter and provide evidence that the age-related susceptibility of DBA/2 mice to audiogenic seizures is related to changes in sensitivity to NMDA receptor-mediated events.