Immunocytochemical localization of GABA and glycine in amacrine and displaced amacrine cells of macaque monkey retina
Vision ResearchPublished 1 December 1993
Margaret A. Koontz, Lisa E. Hendrickson, Sarah Brace, Anita E. Hendrickson
Citations60
SJR quartileQ2
SJR score0.63
SNIP0.89
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TL;DR
The percentage of GABA+ amacrine profiles was higher in central retina than peripheral retina (30-40%), whereas the percentage of GLY+ amACrine profiles did not vary much with eccentricity (52-57%).
Abstract
Recent studies have varied widely in the percentages of GABA- and glycine-immunoreactive (GABA+, GLY+) amacrines reported for primate retina. We compared the distributions of GABA+ and GLY+ amacrines and displaced amacrines at seven locations along the horizontal meridian of macaque retina using postembedding immunogold labeling with silver intensification. The percentage of GABA+ amacrine profiles was higher in central retina (50-55%) than peripheral retina (30-40%), whereas the percentage of GLY+ amacrine profiles did not vary much with eccentricity (52-57%). GABA and glycine were colocalized in 5-20% of amacrines, depending on the eccentricity, whereas 5-30% of amacrines were not immunoreactive for either neurotransmitter. GABA+ amacrines were slightly larger than GLY+ amacrines or Müller cells. In the ganglion cell layer, 5-20% of neurons were labeled for either GABA or glycine and were identified as displaced amacrines. Of these, 53% were GABA+ only, 11% were GLY+ only, and 37% were double-labeled. A few large, very lightly labeled GABA+ cells were identified as ganglion cells. Other features that varied with eccentricity included the linear density of GABA+ and GLY+ amacrines, and the ratio of amacrines to Müller cells.
Keywords
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