The cooperative binding of glucose to yeast hexokinase PI dimer
Biochemical and Biophysical Research CommunicationsPublished 1 October 1976
ELAINE L. TICKNER, J. G. Hoggett, George L. Kellett
Citations7
SJR quartileQ2
SJR score0.75
SNIP0.56
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TL;DR
The possibiely exists therefore that the yeast haxokinase PI and OII isozymes may differ markedly in their regulatory properties.
Abstract
The binding of glucose to yeast hexokinase PI isozyme has been measured by micro scale equilibrium dialysis using a double labeling techniques. It was found that under conditions where the enzyme exists exclusively as dimer glucose bound to one site per monomer with strong cooperatively (Hill coefficient = 1.6). Under the same conditions, the PII isozyme dimer binds glucose non-cooperatively (1). The possibiely exists therefore that the yeast haxokinase PI and OII isozymes may differ markedly in their regulatory properties.
Keywords
MedicineBiochemistry, Genetics and Molecular Biology
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