Hypothalamic melatonin receptor sites revealed by autoradiography
Brain ResearchPublished 1 December 1987
J. Vaněček, A Pavlík, Helena Illnerová
Citations395
SJR quartileQ2
SJR score0.85
SNIP0.72
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TL;DR
125I-Melatonin was used to localize and characterize the melatonin receptor sites in the rat hypothalamus and revealed that displaceable 125I-melatonin binding occurred in suprachiasmatic nuclei and median eminence only.
Abstract
125I-Melatonin was used to localize and characterize the melatonin receptor sites in the rat hypothalamus. Autoradiography revealed that displaceable 125I-melatonin binding occurred in suprachiasmatic nuclei and median eminence only. Further studies performed on crude membrane fractions from median eminences revealed high affinity (Kd = 21 pM) melatonin binding sites (Bmax = 8.5 fmol/mg protein). The order of potency of various indole amines to inhibit 125I-melatonin binding was melatonin much greater than N-acetyl-5-hydroxytryptamine greater than 5-methoxytryptamine greater than 5-hydroxytryptamine.
Keywords
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