Manual and Automated Determination of Immunoglobulins in Unconcentrated Cerebrospinal Fluid
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TL;DR
Values determined by the manual method for determination in human cerebrospinal fluid of the immunoglobulins IgG, IgA, and IgM correlate with values for gamma globulin obtained by electrophoresis.
Abstract
Abstract Manual procedures have been developed for determination in human cerebrospinal fluid of the immunoglobulins IgG, IgA, and IgM. An automated continuous-flow procedure for determination of IgG in human CSF is also described. The immunoglobulins are quantitated in both cases by immunoprecipitin reactions, with use of commercial antiserum. Values determined for the immunoglobulins by the manual method correlate with values for gamma globulin obtained by electrophoresis. The values for IgG from the manual method also correlate with IgG values determined by the automated procedure. The precision of duplicate determinations with the manual procedures for IgG, IgA, and IgM is 3.4, 1.8, and 1.0% (relative standard deviation), respectively, and that for lgG with the automated system is 3.48%.