An Empirical Protocol for Indirect Measurement of Leaf Area Index in Grape (Vitis vinifera L.)

A protocol for using a commercially available instrument to determine leaf area index (LAI) indirectly in a trellised vineyard from knowledge of plant spacing, so that leaf area per vine can be calculated as required.

Abstract

Leaf area development and canopy structure are important characteristics affecting yield and fruit quality of grapevines. Trellising systems and wide row spacing are common viticultural practices that violate key assumptions of currently available indirect methods of leaf area determination. We have developed a protocol for using a commercially available instrument to determine leaf area index (LAI) indirectly in a trellised vineyard. From knowledge of plant spacing, leaf area per vine can be calculated as required. A derived calibration equation resulted in a near 1:1 relationship (y = 0.00 + 1.00 X; r 2 = 0.998) between actual and indirectly determined LAI over a range of LAI induced by irrigation treatments. The protocol involved covering 75% of the sensor with a manufacturer-supplied field of view delimiter and masking data from the outer three (of five) concentric radiation sensors. The protocol could form the basis for a general measurement technique, but may require local calibration.