A radioenzymatic microassay for simultaneous measurement of catecholamines and their deaminated metabolites
Biochemical MedicinePublished 1 December 1979
Nicolas D. Vlachakis, Natalie Alexander, Manuel T. Velasquez, Robert F. Maronde
Citations30
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TL;DR
A simple and specific assay for measurement of norepinephrine, epinephrine, and their major deaminated metabolites 3, 4-dihydroxyphenylglycol and 3,4-dhydroxymandelic acid in plasma has been developed and results can be obtained in less than 3 hr.
Abstract
A simple and specific assay for measurement of norepinephrine, epinephrine, and their major deaminated metabolites 3,4-dihydroxyphenylglycol and 3,4-dihydroxymandelic acid in plasma has been developed. The assay is based on the conversion of these compounds to their O-methylated derivatives in the presence of catechol-O-methyltransferase and S-adenosyl-[methyl3H]methionine. The formed tritiated derivatives are selectively extracted in organic solvents and isolated by thin-layer chromatography. After oxidation to vanillin they are purified by solvent extraction and measured by liquid scintillation spectrophotometry. The assay is rapid and results can be obtained in less than 3 hr.
Keywords
Chemistry
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