Hypersensitive site 4 of the human β globin locus control region
Nucleic Acids ResearchPublished 1 January 1991Open access
Sara Pruzina, O Hanscombe, David Whyatt, Frank Grosveld, Sjaak Philipsen
Citations136
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TL;DR
Bandshift analysis shows that the footprints of HSS4 correspond to binding sites for the erythroid specific proteins GATA1 and NF-E2 and a number of ubiquitous proteins, including jun/fos, Sp1 and TEF2, which shows the role of the individual hypersensitive sites of the LCR.
Abstract
The Locus Control Region (LCR) of the human beta globin gene domain is defined by four erythroid-specific DNasel hypersensitive sites (HSS) located upstream of this multigene cluster. The LCR confers copy number dependent high levels of erythroid specific expression to a linked transgene, independent of the site of integration. To assess the role of the individual hypersensitive sites of the LCR, we have localized HSS4 to a 280bp fragment that is functional both in murine erythroleukaemia (MEL) cells and in transgenic mice. This fragment coincides with the major area of hypersensitivity 'in vivo' and contains a number of DNasel footprints. Bandshift analysis shows that these footprints correspond to binding sites for the erythroid specific proteins GATA1 and NF-E2 and a number of ubiquitous proteins, including jun/fos, Sp1 and TEF2.
Keywords
Biochemistry, Genetics and Molecular Biology
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