Frequency and Distribution of DNA Uptake Signal Sequences in the <i>Haemophilus influenzae</i> Rd Genome
SciencePublished 28 July 1995
Hamilton O. Smith, Jean-François Tomb, Brian Dougherty, Robert Fleischmann, J. Craig Venter
Citations218
SJR quartileQ1
SJR score10.42
SNIP6.62
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TL;DR
The naturally transformable, Gram-negative bacterium Haemophilus influenzae Rd preferentially takes up DNA of its own species by recognizing a 9-base pair sequence, 5'-AAGTGCGGT, carried in multiple copies in its chromosome.
Abstract
The naturally transformable, Gram-negative bacterium Haemophilus influenzae Rd preferentially takes up DNA of its own species by recognizing a 9-base pair sequence, 5'-AAGTGCGGT, carried in multiple copies in its chromosome. With the availability of the complete genome sequence, 1465 copies of the 9-base pair uptake site have been identified. Alignment of these sites unexpectedly reveals an extended consensus region of 29 base pairs containing the core 9-base pair region and two downstream 6-base pair A/T-rich regions, each spaced about one helix turn apart. Seventeen percent of the sites are in inverted repeat pairs, many of which are located downstream to gene termini and are capable of forming stem-loop structures in messenger RNA that might function as signals for transcription termination.
Keywords
Biochemistry, Genetics and Molecular BiologyEnvironmental Science
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