Structure-independent nucleotide sequence analysis.
Proceedings of the National Academy of SciencesPublished 1 May 1979Open access
D. R. Mills, Fred Russell Kramer
Citations193
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TL;DR
Inosine substitution for granosine should markedly improve the resolution that can be obtained in the sequencing of DNA as well as RNA.
Abstract
Substitution of inosine for granosine in the nucleic acid fragments synthesized for the sequencing of RNA effectively prevents the formation of secondary structures during electrophoretic analysis. Consequently, the mobility of each fragment in the sequencing gel is a strict function of its molecular weight. Inosine substitution should markedly improve the resolution that can be obtained in the sequencing of DNA as well as RNA.
Keywords
Biochemistry, Genetics and Molecular Biology
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