IKK-1 and IKK-2: Cytokine-Activated IκB Kinases Essential for NF-κB Activation
SciencePublished 31 October 1997
Frank Mercurio, Hengyi Zhu, Brion W. Murray, Andrej Shevchenko, Brydon L. Bennett, Jian Wu Li
Citations2,043
SJR quartileQ1
SJR score10.42
SNIP6.62
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Abstract
Activation of the transcription factor nuclear factor kappa B (NF-kappaB) is controlled by sequential phosphorylation, ubiquitination, and degradation of its inhibitory subunit IkappaB. A large multiprotein complex, the IkappaB kinase (IKK) signalsome, was purified from HeLa cells and found to contain a cytokine-inducible IkappaB kinase activity that phosphorylates IkappaB-alpha and IkappaB-beta. Two components of the IKK signalsome, IKK-1 and IKK-2, were identified as closely related protein serine kinases containing leucine zipper and helix-loop-helix protein interaction motifs. Mutant versions of IKK-2 had pronounced effects on RelA nuclear translocation and NF-kappaB-dependent reporter activity, consistent with a critical role for the IKK kinases in the NF-kappaB signaling pathway.
Keywords
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