Sex Differences in the Effects of Acute Swim Stress on Binding to GABA_A Receptors in Mouse Brain

Changes in the characteristics of GABA binding to membranes prepared from the forebrain were larger when measured in a relatively crude membrane preparation than in a well‐washed membrane preparation commonly used in GABA binding assays, consistent with the loss of endogenous modulators of GABAbinding in the latter preparation.

Abstract

Acute swim stress (3-min swim at 32 degrees C) in female, but not in male, mice results in substantial changes in the characteristics of GABA binding to membranes prepared from the forebrain. These changes were larger when measured in a relatively crude membrane preparation than in a well-washed membrane preparation commonly used in GABA binding assays, consistent with the loss of endogenous modulators of GABA binding in the latter preparation. These changes may be related to stress-induced alterations in part in the modulation of the characteristics of GABA binding by endogenous steroids, as the acute swim stress produced a larger increase in plasma corticosterone levels in female than in male mice.