Sequential expression of murine homeo box genes during F9 EC cell differentiation.
The EMBO JournalPublished 1 September 1986Open access
Georg Breier, Maja Bućan, Uta Francke, Anamaris M. Colberg‐Poley, P. Gruss
Citations107
SJR quartileQ1
SJR score4.82
SNIP1.93
Generate an AI Snapshot to get a quick, structured summary of this paper.
Study Snapshot
ObjectiveStudy objective
MethodsResearch methodology
PopulationPopulation studied
Sample sizeSample sizes
OutcomesStudy outcomes here
ResultsStudy results comes here
LimitationsResearch study limitations comes here
A concise AI-generated summary of the paper will appear here once you click Generate AI Snapshot.
TL;DR
A previously unknown member of the murine homeo box family is isolated and characterized and it is shown that this gene encodes a 2.7 kb mRNA which is expressed during mouse embryogenesis and during differentiation of F9 teratocarcinoma cells into parietal endoderm cells.
Abstract
We have isolated and characterized a previously unknown member of the murine homeo box family. The new locus, m31, is located on chromosome 15 and is more homologous to sequences contained in the Drosophila homeotic gene, Antp, than to any other known murine homeo box. We show that this gene encodes a 2.7 kb mRNA which is expressed during mouse embryogenesis and during differentiation of F9 teratocarcinoma cells into parietal endoderm cells. The transcript appears late in this differentiation process and is most abundant at a time when the F9 cells begin to express tissue-specific markers and the expression of another murine homeo box gene, m6, has decreased.
Keywords
Biochemistry, Genetics and Molecular Biology
Proceedings of the National Academy of SciencesDNA sequencing with chain-terminating inhibitors
69,306 Citations1977Frederick Sanger, S. Nicklen +1 more
A new method for determining nucleotide sequences in DNA is described, which makes use of the 2',3'-dideoxy and arabinon nucleoside analogues of the normal deoxynucleoside triphosphates, which act as specific chain-terminating inhibitors of DNA polymerase.
Analytical BiochemistryA technique for radiolabeling DNA restriction endonuclease fragments to high specific activity
25,157 Citations1983Andrew P. Feinberg, Bert Vogelstein
A technique for conveniently radiolabeling DNA restriction endonuclease fragments to high specific activity is described, and these "oligolabeled" DNA fragments serve as efficient probes in filter hybridization experiments.
BiochemistryIsolation of biologically active ribonucleic acid from sources enriched in ribonuclease
22,203 Citations1979John M. Chirgwin, Alan Przybyla +2 more
Intact ribonucleic acid has been prepared from tissues rich in ribonuclease such as the rat pancreas by efficient homogenization in a 4 M solution of the potent protein denaturant guanidinium thiocyanate plus 0.1 M 2-mercaptoethanol to break protein disulfide bonds.
Proceedings of the National Academy of SciencesGenomic sequencing.
8,227 Citations1984George M. Church, W Gilbert
The genomic sequencing procedures are applicable to the analysis of genetic polymorphisms, DNA methylation at deoxycytidines, and nucleic acid-protein interactions at single nucleotide resolution.
Journal of Molecular BiologyLambda replacement vectors carrying polylinker sequences
1,753 Citations1983Anna‐Maria Frischauf, Hans Lehrach +2 more
A new family of lambda replacement vectors and derivatives containing amber mutations and derivatives of the EMBL3 vector allow the application of genetic screening procedures based on selection for the products of homologous recombination events, and for the selective cloning of DNA sequences linked to supF genes.
NatureA conserved DNA sequence in homoeotic genes of the Drosophila Antennapedia and bithorax complexes
1,085 Citations1984William McGinnis, Michael Levine +3 more
A repetitive DNA sequence has been identified in the Drosophila melanogaster genome that appears to be localized specifically within genes of the bithorax and Antennapedia complexes that are required for correct segmental development.
CellA homologous protein-coding sequence in drosophila homeotic genes and its conservation in other metazoans
951 Citations1984William McGinnis, Richard L. Garber +3 more
It is shown that this DNA homology (the homeo box) is due to a conserved protein-coding sequence present in these three pattern-formation genes and is reflected in a structural homology in their gene products.
CellHormonal induction of differentiation in teratocarcinoma stem cells: Generation of parietal endoderm by retinoic acid and dibutyryl cAMP
801 Citations1980Sidney Strickland, Karen Kaye Smith +1 more
The cell type generated by retinoic acid and dibutyryl cAMP treatment is therefore indistinguishable from definitive parietal endoderm, and analysis of the final phenotype indicates that it is not dependent upon the continued presence of either compound, and that cAMP agents are active only on cells that have been treated with retinoi acid.
NatureSequence of a Drosophila segmentation gene: protein structure homology with DNA-binding proteins
678 Citations1984Allen Laughon, Matthew P. Scott
Mutations in the fushi tarazu (ftz) locus of Drosophila result in embryos with half the usual number of body segments, and a portion of the conserved protein domain present in ftz and several homoeotic genes resembles the DNA- binding region of prokaryotic DNA-binding proteins.
Proceedings of the National Academy of SciencesAlkaline Phosphatase Activity in Mouse Teratoma
628 Citations1973Edward G. Bernstine, Martin Hooper +2 more
The embryonal carcinoma enzyme resembles the enzymes from kidney and placenta in kinetics of thermal inactivation and sensitivity to the inhibitor L-phenylalanine, but is distinguishable from the alkaline phosphatases of liver and intestine.
NatureEmbryonic lethal mutation in mice induced by retrovirus insertion into the α1(I) collagen gene
422 Citations1983Angelika Schnieke, Klaus Harbers +1 more
Experimental insertion of a retrovirus into the germ line of mice has resulted in an embryonic recessive lethal mutation at the 5' end of the alpha 1(I) collagen gene, leading to complete transcriptional block.
CellDevelopment of embryonic pattern in D. melanogaster as revealed by accumulation of the nuclear engrailed protein
404 Citations1985Stephen DiNardo
The complex final form of the head and terminalia derive from earlier simple subdivision of these areas into developmental fields by engrailed, suggesting that engrailing may be responding to pair-rule segmentation gene products.
NatureThe Drosophila developmental gene, engrailed, encodes a sequence-specific DNA binding activity
384 Citations1985Claude Desplan, James F. Theis +1 more
Plasmid expression vectors carrying either the entire engrailed coding region or a subfragment including the homoeo box, produce protein fusions having sequence-specific DNA binding activity.
NatureFly and frog homoeo domains show homologies with yeast mating type regulatory proteins
344 Citations1984John C.W. Shepherd, William McGinnis +3 more
A search made in the Dayhoff sequence bank reveals a lesser but apparently significant homology between the homoeo domain and the amino acids coded from parts of the a1 and α2 mating type genes of the yeast Saccharomyces cerevisiae.
CellMolecular cloning and chromosome mapping of a mouse DNA sequence homologous to homeotic genes of drosophila
316 Citations1984William McGinnis, Charles P. Hart +2 more
CellLocalization of the fushi tarazu protein during Drosophila embryogenesis
293 Citations1985Susan C. Carroll
The fushi tarazu (ftz) gene of Drosophila acts early in embryogenesis to regulate body segmentation and at the later stage, ftz is expressed in each of fifteen metameric subunits of the embryo.
NatureAn inherited limb deformity created by insertional mutagenesis in a transgenic mouse
291 Citations1985Richard P. Woychik, Timothy A. Stewart +3 more
An insertional mutation is created that leads to a severe defect in the pattern of limb formation in the developing mouse, and is closely linked to a dominant mutation that gives rise to a related limb dysmorphism.
The EMBO JournalGenomic and cDNA clones of the homeotic locus Antennapedia in Drosophila.
287 Citations1983Richard L. Garber, Atsushi Kuroiwa +1 more
Using a combination of chromosome walking and jumping, a DNA region from Drosophila containing Antennapedia is cloned, and the unusual size and complexity of this locus are discussed.
Biochemical and Biophysical Research CommunicationsAlkaline transfer of DNA to plastic membrane
285 Citations1984Piotr Chomczyński, Pradman K. Qasba
The alkaline transfer offers a higher hybridization efficiency and simplifies the transfer procedure as compared with the standard method of DNA transfer at neutral pH, and can be used to remove RNA contamination from the transferred DNA.
NaturePost-transcriptional control of myc and p53 expression during differentiation of the embryonal carcinoma cell line F9
270 Citations1985Carola Dony, Michael Kessel +1 more
It is reported that down-regulation of stable myc and p53 RNA during irreversible differentiation of F9 cells occurs at the post-transcriptional level.
CellRegulation of the Ultrabithorax gene of drosophila by other bithorax complex genes
255 Citations1985Gary Struhl, Robert A. White
It is shown that distinct patterns of expression within the bithorax complex of Drosophila result in large part from regulation of Ubx gene expression by the abd-A and Abd-B gene functions.
GeneRapid restriction mapping of DNA cloned in lambda phage vectors
249 Citations1984Hans‐Richard Rackwitz, Günther Zehetner +2 more
A protocol for the rapid restriction mapping of phage lambda clones has been developed and the restriction map can be directly determined from the "ladder" of partial digestion products.
The EMBO JournalDistribution of Ultrabithorax proteins in Drosophila
246 Citations1985Robert A. White, Michael Wilcox
The distribution of Ultrabithorax (Ubx) proteins in Drosophila embryos and imaginal discs by immunofluorescence is examined using a monoclonal antibody to examine a repeat unit, the Ubx metamere, out of phase with the segmental repeat unit.
NatureRegulation of Antennapedia transcript distribution by the bithorax complex in Drosophila
244 Citations1984Ernst Hafen, Michael Levine +1 more
In situ hybridization of an Antp+ cDNA probe to tissue sections of embryos, it is found that the neural cells of the transformed segments accumulateAntp+ transcripts to a level characteristic of the normal mesothorax.
The EMBO JournalIsolation of caudal, a Drosophila homeo box‐containing gene with maternal expression, whose transcripts form a concentration gradient at the pre‐blastoderm stage
242 Citations1985Marek Mlodzik, Anders Fjose +1 more
CellExpression during embryogenesis of a mouse gene with sequence homology to the Drosophila engrailed gene
233 Citations1985Alexandra L. Joyner
Analysis of poly(A)+ RNA extracted from teratocarcinoma cells and whole mouse embryos demonstrates that the conserved homeo box region of Mo-en.1 is expressed differentially during mouse embryogenesis, indicating a mouse homolog of a gene of the Drosophila engrailed gene complex.
CellHuman DNA sequences homologous to a protein coding region conserved between homeotic genes of Drosophila
222 Citations1984Michael Levine, Gerald M. Rubin +1 more
NatureStructural analysis of murine genes containing homoeo box sequences and their expression in embryonal carcinoma cells
209 Citations1985Anamaris M. Colberg‐Poley, Stephan D. Voss +2 more
A novel murine homoeo box-containing gene is expressed in embryonal carcinoma stem cells and Transcripts of the sequences that flank the homoea box of this gene are found in these cells before and after induced differentiation, whereas a specific transcript that seems to contain the homOEo sequence is only present after differentiation.
NatureSpatial restriction in expression of a mouse homoeo box locus within the central nervous system
202 Citations1986Alexander Awgulewitsch, Manuel F. Utset +3 more
Using Northern blot analysis and in situ hybridization to mouse tissue sections, the spatially restricted expression of a newly identified mouse homoeo box locus, Hox-3, within the central nervous system of newborn and adult mice has been demonstrated.
CellGenetic analysis of the proximal portion of the mouse t complex: Evidence for a second inversion within t haplotypes
194 Citations1986Bernhard G. Herrmann, Marija Bućan +4 more
The existence of a large inversion of genetic material, encompassing the loci of T and qk, within the proximal portion of t haplotypes, provides an explanation for the suppression of recombination observed along the length of t Haplotype DNA in heterozygous mice.
CellClustered homeo boxes are differentially expressed during murine development
172 Citations1985Anamaris M. Colberg‐Poley, Stephan D. Voss +4 more
A murine homeo box known to be expressed during differentiation of embryonal carcinoma cells lies within a cluster of homeo boxes located in 30 kilobase pairs of genomic DNA, and the differential expression of these homeo-box-containing regions in various adult tissues is described.
Proceedings of the National Academy of SciencesIsolation of cDNA clones specific for collagen IV and laminin from mouse teratocarcinoma cells.
163 Citations1983S Y Wang, Lorraine J. Gudas
Time course of the increase in mRNA coding for collagen IV (alpha 2) and laminin B in F9 cells after retinoic acid and Bt2cAMP treatment is studied, with a dramatic increase between 12 and 24 hr after drug treatment.
CellHomeo box gene complex on mouse chromosome 11: Molecular cloning, expression in embryogenesis, and homology to a human homeo box locus
155 Citations1985Charles P. Hart
Cloned three overlapping recombinant phage clones that cover a region of mouse chromosome 11 that contains a cluster of four homeo boxes encodes multiple transcripts that are expressed during embryogenesis provide direct evidence for genetic expression during embryonic development and a conserved organization in comparison to the cognate human locus.
Cytogenetics and Cell GeneticsGene mapping in Mus musculusby interspecific cell hybridization: assignment of the genes for tripeptidase-1 to chromosome 10, dipeptidase-2 to chromosome 18, acid phosphatase-1 to chromosome 12, and adenylate kinase-1 to chromosome 2
140 Citations2008Uta Francke, P.A. Lalley +3 more
The cytologic studies provide a comparison between data from sexual genetics and somatic cell hybrids and validate hybrid cell techniques and provide evidence of the reliability of scoring chromosomes by GTG and Hoechst staining and stress the importance of identifying clones with multiple chromosome rearrangements.
CellCloning and transcriptional analysis of the segmentation gene fushi tarazu of Drosophila
135 Citations1984Atsushi Kuroiwa, Ernst Hafen +1 more
The ftz gene encodes a single 1.9 kb poly(A)+ RNA expressed exclusively from the early blastoderm to gastrula stages of embryonic development and has been analyzed by S1 nuclease mapping and by restriction mapping of a cDNA clone.
CellExpression of homologous homeo-box-containing genes in differentiated human teratocarcinoma cells and mouse embryos
128 Citations1985Craig A. Hauser
It is found that a human teratocarcinoma cell line expresses high levels of Hu1 homeo-box-containing mRNA only after differentiation of the cells following treatment with retinoic acid, whereas the expression of such transcripts was detected in mouse embryos from 10 to 17 days of gestation.
NatureA mouse homoeo box gene is expressed during embryogenesis and in adult kidney
117 Citations1985Ian J. Jackson, Paul N. Schofield +1 more
A sensitive RNase protection assay is used to examine transcription of the H24.1 gene during mouse development, and in adult tissues, to report that the gene is transcribed from as early as 7.5 days post coitum (p.c.), with maximum expression at days 11.5 and 12.5 p.c.
CellA homeo-box-containing gene expressed during oogenesis in xenopus
113 Citations1984Michael M. Müller, Andrés E. Carrasco +1 more
The main interest of this gene lies in the fact that it is abundantly transcribed in large Xenopus oocytes, where it might be translated into proteins potentially involved in the early determination of embryonic cell types.
Proceedings of the National Academy of SciencesAssignment of the gene for cytoplasmic superoxide dismutase ( <i>Sod-1</i> ) to a region of chromosome 16 and of <i>Hprt</i> to a region of the X chromosome in the mouse
99 Citations1979Uta Francke, R.T. Taggart
Using a set of 29 mouse-Chinese hamster somatic cell hybrids, the locus for SOD-1 is assigned to the distal half of mouse chromosome 16 and part of the mouse X chromosome carrying the complementing Hprt gene can be identified by selection in hypoxanthine/aminopterin/thymidine medium and counterselection in 8-azaguanine.
Nucleic Acids ResearchIsolation of cDNA clones for basal lamina components: type IV procollagen
93 Citations1983Markku Kurkinen, Denise P. Barlow +3 more
It is shown that an increase in cellular concentration of type IV procollagen mRNA occurs within 24 to 48 hours of induction, reaching a constant high level by 72 hours.
NatureComparative chromosome mapping of a conserved homoeo box region in mouse and human
92 Citations1985Alexandra L. Joyner, R V Lebo +4 more
The localization of a human homoeo box region, previously cloned and shown to contain two homoea boxes within a sequence of 5-kilobases (kb), to the long arm of chromosome 17 is reported and data suggest that the homoee box region detected with probes is highly conserved in human and mouse.
The EMBO JournalCloning of the homeotic Sex combs reduced gene in Drosophila and in situ localization of its transcripts
87 Citations1985Atsushi Kuroiwa, Urs Kloter +2 more
The ‘chromosomal walk’ in the Antennapedia‐complex (ANT‐C) is extended by isolating overlapping DNA sequences spanning the chromosomal segment between Antp and Deformed and mapping the transcription units, homeoboxes and M‐repeats within this region.
NatureTwo homoeo box loci mapped in evolutionarily related mouse and human chromosomes
86 Citations1985Mark Rabin, Charles P. Hart +4 more
Experiments are described which show that two homoeo box loci map, respectively, to evolutionarily related regions on mouse chromosome 11 and human chromosome 17.
GeneMolecular cloning of DNA complementary to a mouse α-fetoprotein mRNA sequence
35 Citations1980S W Law, Taiki Tamoaki +2 more
DNA complementary to mouse yolk sac messenger RNA has been inserted at the PstI site of the plasmid pBR322 by annealing of the oligo(dG)-tailed plasmids DNA with the oligO(dC)-tailed mouse DNA.