Analysis of passive and light-driven ion movements in large bacteriorhodopsin liposomes reconstituted by reverse-phase evaporation. 2. Influence of passive permeability and back-pressure effects upon light-induced proton uptake
BiochemistryPublished 1 October 1986
Michel Seigneuret, Jean Louis Rigaud
Citations32
SJR quartileQ1
SJR score1.18
SNIP0.73
Generate an AI Snapshot to get a quick, structured summary of this paper.
Study Snapshot
ObjectiveStudy objective
MethodsResearch methodology
PopulationPopulation studied
Sample sizeSample sizes
OutcomesStudy outcomes here
ResultsStudy results comes here
LimitationsResearch study limitations comes here
A concise AI-generated summary of the paper will appear here once you click Generate AI Snapshot.
Abstract
ADVERTISEMENT RETURN TO ISSUEPREVArticleAnalysis of passive and light-driven ion movements in large bacteriorhodopsin liposomes reconstituted by reverse-phase evaporation. 2. Influence of passive permeability and back-pressure effects upon light-induced proton uptakeMichel Seigneuret and Jean Louis RigaudCite this: Biochemistry 1986, 25, 21, 6723–6730Publication Date (Print):October 1, 1986Publication History Published online1 May 2002Published inissue 1 October 1986https://pubs.acs.org/doi/10.1021/bi00369a060https://doi.org/10.1021/bi00369a060research-articleACS PublicationsRequest reuse permissionsArticle Views214Altmetric-Citations28LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
Keywords
NeuroscienceBiochemistry, Genetics and Molecular BiologyPhysics and Astronomy
Journal of the American Oil Chemists SocietyChromatographically homogeneous lecithin from egg phospholipids
1,302 Citations1965W. S. Singleton, M. S. Gray +2 more
Journal of Biological ChemistryBinding of Diffusible Molecules by Macromolecules: Rapid Measurement by Rate of Dialysis
516 Citations1969Sidney P. Colowick, Frances C. Womack
A rapid technique for the determination of the dissociation constant and the number of binding sites of an enzyme-substrate complex has been developed based on measuring the rate of dialysis of radioactive substrate from an enzyme -substrate equilibrium mixture.
Biochemical and Biophysical Research CommunicationsIncorporation of bacteriorhodopsin into large unilamellar liposomes by reverse phase evaporation
117 Citations1983Jean Louis Rigaud, Aline Bluzat +1 more
Electron microscopy showed that proteoliposomes were unilamellar and fairly uniform in size provided the preparation was extruded through calibrated nucleopore membranes : the vesicles have diameters around 200 nm.
Biochimica et Biophysica Acta (BBA) - BioenergeticsBacteriorhodopsin in liposomes. II. Experimental evidence in support of a theoretical model
85 Citations1979Klaas J. Hellingwerf, Jos C. Arents +2 more
In three independent experimental systems evidence was obtained for the correctness of the postulated dependence of the turnover rate of the photochemical cycle on back pressure by the transmembrane electrochemical potential difference for protons.
BiochemistryPhospholipase D from Savoy cabbage: purification and preliminary kinetic characterization
74 Citations1979Thomas T. Allgyer, Michael A. Wells
Phospholipase D has been purified 680-fold from an acetone powder of savoy cabbage in an overall yield of 30% on the basis of gel electrophoresis and ultracentrifugation.
Journal of Biological ChemistryReconstitution of delipidated bacteriorhodopsin with endogenous polar lipids.
72 Citations1981Christina Lind, B. Höjeberg +1 more
Bacteriorhodopsin in the reconstituted vesicles has an inside out orientation and, on illumination, translocates protons efficiently from the medium to the interior of the vesicle in the presence of the ionophore valinomycin.
FEBS LettersUse of the fluorescent pH probe pyranine to detect heterogeneous directions of proton movement in bacteriorhodopsin reconstituted large liposomes
57 Citations1985Michel Seigneuret, Jean‐Louis Rigaud
The use of pyranine at acidic pH allowed us to detect two distinct populations of proteoliposomes which exhibited oppositely directed proton pumping, which suggests that the two orientations, of bacteriorhodopsin are at least partially distributed in different proteolIPosomes.
Trends in Biochemical SciencesKeeping a light-driven proton pump under control
48 Citations1984Hans V. Westerhoff, Z. Dancsházy
Biochimica et Biophysica Acta (BBA) - BioenergeticsBacteriorhodopsin in liposomes. I. A description using irreversible thermodynamics
43 Citations1979Hans V. Westerhoff, Bob J. Scholte +1 more
A comprehensive description of light-induced ion transport in bacteriorhodopsin liposomes is presented and rigid trials to falsify these expressions are shown to be unsuccessful.
BiochemistryLinear relations between proton current and pH gradient in bacteriorhodopsin liposomes
34 Citations1981Jos C. Arents, Herman van Dekken +2 more
Since bacteriorhodopsin liposomes are a model system for all biological energy transducing systems in which proton pumps are involved, these findings also explain linear relations between proton flux and protonmotive force observed in and postulated for those systems.
Proceedings of the National Academy of SciencesMosaic nonequilibrium thermodynamics describes biological energy transduction.
34 Citations1981Hans V. Westerhoff, Klaas J. Hellingwerf +3 more
It is concluded that mosaic nonequilibrium thermodynamics describes ion movement and energy transduction in the model system of bacteriorhodopsin liposomes and, therefore, may be applied to any other biological system performing such processes.
Biochimica et Biophysica Acta (BBA) - BiomembranesBacteriorhodopsin vesicles. An outline of the requirements for light-dependent H+ pumping
25 Citations1978Klaas J. Hellingwerf, Bob J. Scholte +1 more
It is concluded that reconstitution of an active light-driven proton pump is possible over a wide range of conditions and most likely, monomeric bacteriorhodopsin molecules can effectively pump protons.
Biochimica et Biophysica Acta (BBA) - BioenergeticsLight dependence of the decay of the proton gradient in broken chloroplasts
24 Citations1979Yee-Kin Ho, C.Jason Liu +2 more
The initial rates and steady-state values of proton uptake by broken chloroplasts have been measured as functions of light intensity at various concentrations of chlorophyll, pyocyanine, supporting electrolyte, buffer, as well as pH and temperature andKinetics analysis of the data shows that the rate of decay ofProton gradient due to backward leakage depends on light intensity.
The Journal of Membrane BiologyBacteriorhodopsin in liposomes: Quantitative evaluation of ΔpH changes induced by variations of light intensity and conductivity parameters
15 Citations1981Jos C. Arents, Klaas J. Hellingwerf +2 more
A description of ion movement and energy transduction in terms of a kinetic variant of nonequilibrium thermodynamics was subjected to experimental tests in bacteriorhodopsin liposomes and it is suggested that the theoretical description will be useful in other, more complex systems to gain detailed information about their energytransduction and ion permeation properties.
Photochemistry and PhotobiologyA MICROSECOND KINETIC STUDY OF THE PHOTOGENERATED MEMBRANE POTENTIAL OF BACTERIORHODOPSIN WITH A FAST RESPONDING DYE
14 Citations1984Benjamin Ehrenberg, Zvi Meiri +1 more
A dye whose fluorescence intensity responds rapidly to membrane potential was used to follow the evolution of the potential on liposomes reconstituted with bacteriorhodopsin, in the microseconds time domain.
Biochimica et Biophysica Acta (BBA) - BioenergeticsThe effect of ionophores and light intensity on the initial rate of proton uptake into bacteriorhodopsin liposomes can be quantitatively described by mosaic non-equilibrium thermodynamics
14 Citations1981Hans V. Westerhoff, Jos C. Arents +1 more
The analysis showed that bacteriorhodopsin is inhibited by the membrane potential it develops, and the results are in strict, quantitative agreement with the predictions of a theoretical description of bacteriorodopsin liposomes based on mosaic non-equilibrium thermodynamics.
Archives of Biochemistry and BiophysicsProton movement in reconstituted purple membrane of Halobacteria: Effects of pH and ionic composition of the medium
11 Citations1983Fausto Ramírez, Hiroshi Okazaki +2 more
Results and previous work from this laboratory, including the modification of the light-dependent proton-pumping inhibition by the charge of the bilayer, support a picture in which there is an indirect link between the primary proton -pumping activity and its associated inhibitory process in bacteriorhodopsin.
Biophysical JournalCoupling between the bacteriorhodopsin photocycle and the protonmotive force in Halobacterium halobium cell envelope vesicles. III. Time-resolved increase in the transmembrane electric potential and modeling of the associated ion fluxes
11 Citations1985Sam Helgerson, M.K. Mathew +4 more
The results are modeled using an irreversible thermodynamics formulation, which assumes a constant driving reaction affinity and a variable reaction rate for the proton-pumping cycle of bacteriorhodopsin and includes a voltage-gated, electrogenic Na+/H+ antiporter that is active when vesicles are suspended in NaCl.