Preparative High-Performance Liquid Chromatographic Separation of Individual Amino Acids Derived from Fossil Bone Collagen
RadiocarbonPublished 1 January 1990Open access
G. J. VAN KLINKEN, Willem G. Mook
Citations47
SJR quartileQ1
SJR score0.91
SNIP1.40
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TL;DR
The routine high-performance liquid chromatographic extraction of five amino acids: glycine, hydroxyproline, glutamate, threonine and alanine is described, which makes it possible to compare chemically well-characterized molecules from one bone sample, both chemically and by isotopic analysis.
Abstract
Extracting a series of amino acids from fossil “collagen” makes it possible to compare chemically well-characterized molecules from one bone sample, both chemically and by isotopic analysis. Thus, the integrity of collagen samples and possible contamination can be checked. We describe the routine high-performance liquid chromatographic extraction of five amino acids: glycine, hydroxyproline, glutamate, threonine and alanine. Humates are eliminated during the extraction, which can be monitored continuously.
Keywords
Social SciencesEarth and Planetary SciencesEnvironmental Science
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