Deletion mapping of DNA markers to a region of chromosome 5 that cosegregates with schizophrenia
GenomicsPublished 1 November 1989Open access
T. Conrad Gilliam, Nelson B. Freimer, Charles A. Kaufmann, Peter Powchik, Anne S. Bassett, Ulla Bengtsson
Citations45
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TL;DR
A human:hamster hybrid cell line whose only human complement is a chromosome 5 that is missing the trisomic region associated with schizophrenia and the hybrid cell lines HHW 105 and HHW 1064 together provide a means to identify and generate syntenic DNA markers to further investigate the location of a schizophrenia locus.
Abstract
Two independent lines of evidence support the localization of a schizophrenia susceptibility locus to the proximal long arm of chromosome 5. A partial trisomy of chromosome 5 (5q11.2-q13.3) cosegregates with the disorder in a Canadian family of Chinese descent, and DNA markers from proximal 5q cosegregate with schizophrenia (plus related disorders) in families of British and Icelandic descent. We constructed a human:hamster hybrid cell line (HHW 1064) whose only human complement is a chromosome 5 that is missing the trisomic region associated with schizophrenia. In combination with a "matched" cell hybrid (HHW 105) containing an intact chromosome 5, we physically mapped DNA markers relative to the trisomy. "Schizophrenia-linked" DNA markers p105-153Ra (D5S39) and p105-599Ha (D5S76) map within the trisomy and proximal to the 5q11.2 breakpoint, respectively. The hybrid cell lines HHW 105 and HHW 1064 together provide a means to identify and generate syntenic DNA markers to further investigate the location of a schizophrenia locus.
Keywords
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