Edinger-Westphal neurons that project to spinal cord contain substance P
Neuroscience LettersPublished 1 April 1983
B.S. Phipps, R. Maciewicz, Barry B. Sandrew, Charles E. Poletti, Warren E. Foote
Citations45
SJR quartileQ2
SJR score0.77
SNIP0.67
Generate an AI Snapshot to get a quick, structured summary of this paper.
Study Snapshot
ObjectiveStudy objective
MethodsResearch methodology
PopulationPopulation studied
Sample sizeSample sizes
OutcomesStudy outcomes here
ResultsStudy results comes here
LimitationsResearch study limitations comes here
A concise AI-generated summary of the paper will appear here once you click Generate AI Snapshot.
TL;DR
Nearly all HRP-labeled EW neurons also stained for SPLI, evidence that EW is the origin of a direct substance P pathway linking rostral mesencephalon with spinal cord.
Abstract
Combined retrograde transport and immunocytochemical methods were used to determine whether Edinger-Westphal neurons projecting to spinal cord also demonstrate substance P-like immunoreactivity (SPLI). Large injections of horseradish peroxidase (HRP) into cervical and lumbar enlargements retrogradely labeled cells throughout the length of the Edinger-Westphal complex (EW). Nearly all HRP-labeled EW neurons also stained for SPLI, evidence that EW is the origin of a direct substance P pathway linking rostral mesencephalon with spinal cord.
Keywords
MedicineNeuroscience
Journal of Histochemistry & CytochemistryTetramethyl benzidine for horseradish peroxidase neurohistochemistry: a non-carcinogenic blue reaction product with superior sensitivity for visualizing neural afferents and efferents.
4,309 Citations1978M.‐Marsel Mesulam
The sensitivity of this new method in demonstrating retrograde transport is markedly superior to that of the previously described benzidine dihydrochloride method and, as a consequence, many efferent connections of the injection site are also visualized.
Brain ResearchDirect hypothalamo-autonomic connections
1,207 Citations1976Clifford B. Saper, Arthur D. Loewy +2 more
Proceedings of the National Academy of SciencesImmunohistochemical analysis of peptide pathways possibly related to pain and analgesia: enkephalin and substance P.
887 Citations1977Tomas Hökfelt, Å. Ljungdahl +3 more
The present immunohistochemical-anatomical findings support the hypothesis that stimulation-produced analgesia is related to activation of spinal and spinal trigeminal enkephalin interneurons forming axo-axonic synapses with (substance P?) pain afferents in the superficial laminae of the dorsal horn and the spinal trigEMinal nucleus.
Neuroscience LettersRetrograde axonal transport of horseradish peroxidase from spinal cord to brain stem cell groups in the cat
605 Citations1975H.G.J.M. Kuypers, Vladimir A. Maisky
Horseradish peroxidase was injected in cervical, thoracic and lumbar segments of the cat's spinal cord through several transverse rows of narrowly spaced needle penetrations and transported retrogradely to brain stem neurons in the bulbar medial reticular formation, the vestibular complex and the red nucleus.
The Journal of Comparative NeurologyReexamination of the dorsal root projection to the spinal dorsal horn including observations on the differential termination of coarse and fine fibers
465 Citations1979Alan R. Light, Edward R. Perl
Brain ResearchA method based on retrograde intraaxonal transport of protein for identification of cell bodies of origin of axons terminating within the CNS
405 Citations1973Jennifer H. LaVail, Ken R. Winston +1 more
Brain ResearchAfferent projections to the cat locus coeruleus as visualized by the horseradish peroxidase technique
382 Citations1977Kazuya Sakai, Monique Touret +3 more
The present results confirm some previous reports on the afferent connections of the LC described in the rat, cat and rabbit, and further indicate the richness of afferent projections to the dorsolateral pontine tegmental areas.
Brain ResearchSelective reduction by noradrenaline and 5-hydroxytryptamine of nociceptive responses of cat dorsal horn neurones
334 Citations1978P.M. Headley, A.W. Duggan +1 more
Brain ResearchOrigins of serotonergic projections to the spinal cord in rat: An immunocytochemical-retrograde transport study
316 Citations1981Robert M. Bowker, Karin N. Westlund +1 more
Brain ResearchSpinal noradrenergic terminal system mediates antinociception
315 Citations1980S. V. Ramana Reddy, Tony L. Yaksh
The present data provide further evidence for the modulatory role of a spinal noradrenergic system on the spinal processing of nociceptive transmission and no cross-tolerance between intrathecal NE and morphine was observed, suggesting that the spinal action of morphine is not mediated by spinal nordrenergic terminals.
The Journal of Comparative NeurologySpinal projections from the midbrain in monkey
233 Citations1978A. J. Castiglioi, M. C. Gallaway +1 more
Findings indicate that the primate midbrain is the origin of an extensive system of descending spinal pathways, some of which are likely to be involved in mediating descending influences involved in complex motor and sensory behavior.
Brain ResearchDecline in reactive fiber growth in the dentate gyrus of aged rats compared to young adult rats following entorhinal cortex removal
186 Citations1980Stephen W. Scheff, Larry S. Benardo +1 more
Animals in the oldest age group showed at 12 days after the lesion a greater variability in the expansion of the commissural-associational fiber plexus than all younger groups and Astrocytes in the dentate molecular layer appeared to become more abundant and more hypertrophied in unoperated animals with age.
Brain ResearchFunicular trajectories of descending brain stem pathways in cat
185 Citations1977H.G.J.M. Kuypers, Vladimir A. Maisky
The same ipsilateral-contralateral distribution of labeled neurons occurs when the injection at T1 is preceded a week earlier by a contralateral hemisection at C1-C2 (case 13, Fig. 2), which indicates that the fibers from the labeled neurons contralsateral to the injection cross in the brain stem.
Journal of NeuroscienceUltrastructural localization and biochemical features of immunoreactive LEU-enkephalin in monkey dorsal horn
157 Citations1981Neil Aronin, Marian DiFiglia +2 more
The anatomical results suggest that neurons containing immunoreactive Leu-enkephalin in the dorsal horn, some of which may receive input from primary afferents, modulate nociception by directly synapsing with cells of origin of the spinothalamic tract and also by interacting withPrimary afferent terminals.
Brain ResearchEdinger-Westphal nucleus: Projections to the brain stem and spinal cord in the cat
153 Citations1978Arthur D. Loewy, Clifford B. Saper
The traditional view of the Edinger-Westphal nucleus of the cat as merely a parasympathetic preganglionic nucleus should be seriously questioned.
Brain ResearchRe-evaluation of the efferent projections of the Edinger-Westphal nucleus in the cat
124 Citations1978Arthur D. Loewy, Clifford B. Saper +1 more
Journal of Histochemistry & CytochemistryLong-term storage and regular repeated use of diluted antisera in glass staining jars for increased sensitivity, reproducibility, and convenience of single- and two-color light microscopic immunocytochemistry.
108 Citations1982Michael V. Sofroniew, U. Schrell
Diluted antisera, stored at 4 degrees C and continuously reused, were found to be stable for long periods of time, and consistently yielded high quality staining in both single- and two-color immunoperoxidase staining.
Neuroscience LettersDifferential sensitivity between blue and brown reaction procedures for HRP neurohistochemistry
83 Citations1977Marek-Marsel Mesulam, Douglas L. Rosene
It is concluded that the BDHC procedure is more sensitive than a DAB procedure for the demonstration of the retrograde transport of HRP.
Brain ResearchCentral origin of oculomotor parasympathetic neurons in the monkey
76 Citations1980R M Burde, Arthur D. Loewy
Journal of Histochemistry & CytochemistryImmunohistochemical localization of retrogradely and anterogradely transported wheat germ agglutinin (WGA) within the central nervous system of the rat: application to immunostaining of a second antigen within the same neuron.
62 Citations1981Ronald M. Lechan, Jeffrey L. Nestler +1 more
The applicability of this technique to identification of a second antigen within immunoreactive-WGA-labeled neurons is demonstrated by sequential immunostaining of tyrosine hydroxylase within dopamine-containing cells of the mesencephalon, ipsilateral to an injection in the caudate-putamen complex.
Brain ResearchThe distribution of substance P-containing neurons in the cat Edinger-Westphal nucleus: relationship to efferent projection systems
60 Citations1983R. Maciewicz, B.S. Phipps +3 more
The distribution of cells projecting to spinal cord or cerebellum and the pattern of SPLI staining was found to closely overlap, evidence that substance P may be contained in cells that give rise to the central projections of the Edinger--Westphal complex.
Neuroscience LettersDirect projections from the Ediger-Westphal nucleus to the cerebellum and spinal cord in the cat: An HRP study
59 Citations1978Tetsuo Sugimoto, Kazuo Itoh +1 more
A vast majority of neurons in the Edinger-Westphal nucleus (EW), consisting of the anteromedian nucleus (AM) and the posterior part of the EW (EWp: so-called visceral nucleus), were labeled retrogradely with horseradish peroxidase injected into the cerebellum in the cat.
Brain ResearchOn the neuronal origin of the afferents to the ciliary ganglion in cat
54 Citations1980Kiyoaki Toyoshima, Etsuro Kawana +1 more
Brain ResearchLack of unity of Edinger-Westpal nucleus projections to the ciliary ganglion and spinal cord: a double-labeling approach
43 Citations1982Ronald M. Burde, Joseph J. Parelman +1 more