Choline uptake systems of rat brain synaptosomes
Biochimica et Biophysica Acta (BBA) - BiomembranesPublished 1 January 1973
Tatsuya Haga, Haruhiko Noda
Citations390
SJR quartileQ1
SJR score0.81
SNIP0.77
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TL;DR
The results indicate the presence of two carrier-mediated uptake systems for choline, one of which requires Na+ for the uptake and the other does not.
Abstract
The uptake of [3H]choline into synaptosomes and the subsequent synthesis of [3H]acetylcholine were examined as functions of choline, Na+ and hemicholinium-3 concentrations in the incubation medium. The results indicate the presence of two carrier-mediated uptake systems for choline. One system requires Na+ for the uptake and the other does not. About 60% of the choline taken up into synaptosomes by the Na+-dependent system were converted to acetylcholine, whereas only a few percent of the choline taken up into synaptosomes by the Na+-independent system were converted to acetylcholine.Km, the choline concentration giving half-maximum transport was 4–8 μM for the Na+-dependent system and about 40 μM for the Na+-independent system.Ki values for hemicholinium-3 were 0.05–0.1 μM for the Na+-dependent system and 40–50 μM for the Na+-independent system.
Keywords
ChemistryNeuroscience
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