Contiguation of Bacterial Clones
Humana Press eBooksPublished 15 November 2003
Sean Humphray, Susan J. Knaggs, Ioannis Ragoussis
Citations9
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Abstract
AbstractFor accurate assembly of a bacterial clone map (or contig), it is necessary to precisely detect the degree of overlap and map order of clones. One way is to generate fingerprints for all the clones in a given project. The overlap between clones is determined by the proportion of comigrating restriction fragments on either acrylamide or agarose gels, with mulitfold coverage of clones allowing rapid identification of artifacts such as rearranged or deleted clones.KeywordsDeionized Distil WaterBacterial CloneStandard Saline CitrateMarker LaneSaran WrapThese keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.
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