Quantitative mapping of amplicon structure by array CGH identifies CYP24 as a candidate oncogene
Nature GeneticsPublished 1 June 2000
Donna G. Albertson, Bauke Ylstra, Richard Segraves, Colin C. Collins, Shanaz H. Dairkee, David Kowbel
Citations597
SJR quartileQ1
SJR score16.59
SNIP6.64
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TL;DR
It is shown here that quantitative measurement of DNA copy number across amplified regions using array comparative genomic hybridization (CGH) may facilitate oncogene identification by providing precise information on the locations of both amplicon boundaries and amplification maxima.
Abstract
We show here that quantitative measurement of DNA copy number across amplified regions using array comparative genomic hybridization (CGH) may facilitate oncogene identification by providing precise information on the locations of both amplicon boundaries and amplification maxima. Using this analytical capability, we resolved two regions of amplification within an approximately 2-Mb region of recurrent aberration at 20q13.2 in breast cancer. The putative oncogene ZNF217 (ref. 5) mapped to one peak, and CYP24 (encoding vitamin D 24 hydroxylase), whose overexpression is likely to lead to abrogation of growth control mediated by vitamin D, mapped to the other.
Keywords
MedicineBiochemistry, Genetics and Molecular Biology
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